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m6A甲基化分析揭示了一个全同胞美利奴羊家族中粗羊毛和细羊毛性状背后的网络及关键基因。

m6A Methylation Analysis Reveals Networks and Key Genes Underlying the Coarse and Fine Wool Traits in a Full-sib Merino Family.

作者信息

Hua Guoying, Yang Xue, Ma Yuhao, Li Tun, Wang Jiankui, Deng Xuemei

机构信息

Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, China & Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing 100193, China.

出版信息

Biology (Basel). 2022 Nov 9;11(11):1637. doi: 10.3390/biology11111637.

DOI:10.3390/biology11111637
PMID:36358338
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9687456/
Abstract

In our study, a set of lambs with coarse wool type all over their bodies were discovered within a full-sib family during an embryo transfer experiment of merino fine wool sheep. The difference between coarse and fine wool traits were studied from the perspective of RNA modification-N6-methyladenosine. A total of 31,153 peaks were collected, including 15,968 peaks in coarse skin samples and 15,185 peaks in fine skin samples. In addition, 7208 genes were differentially m6A methylated, including 4167 upregulated and 3041 downregulated in coarse skin samples. Four key genes (, , , ) were obtained by comprehensive analysis of the MeRIP-seq and RNA sequence, which are closely related to primary wool follicle morphogenesis and development. The PI3K/AKT pathway was enriched through different m6A-related genes. These results provided new insights to understand the role of epigenetics in wool sheep domestication and breeding.

摘要

在我们的研究中，在美利奴细毛羊胚胎移植实验期间，于一个全同胞家系中发现了一组全身为粗毛型的羔羊。从RNA修饰-N6-甲基腺苷的角度研究了粗毛和细毛性状之间的差异。共收集到31,153个峰，其中粗皮样品中有15,968个峰，细皮样品中有15,185个峰。此外，有7208个基因发生了m6A甲基化差异，其中粗皮样品中有4167个上调，3041个下调。通过对MeRIP-seq和RNA序列的综合分析获得了四个关键基因（，，，），它们与初级毛囊的形态发生和发育密切相关。PI3K/AKT通路通过不同的m6A相关基因得以富集。这些结果为理解表观遗传学在毛用绵羊驯化和育种中的作用提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/0102527b295b/biology-11-01637-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/b3d98643d824/biology-11-01637-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/ba5e087d066e/biology-11-01637-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/b3a829583b27/biology-11-01637-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/36e2fe0006c3/biology-11-01637-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/2e86f24adb61/biology-11-01637-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/783e5fa8e9ba/biology-11-01637-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/0102527b295b/biology-11-01637-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/b3d98643d824/biology-11-01637-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/6f04280f19e1/biology-11-01637-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/ba5e087d066e/biology-11-01637-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/b3a829583b27/biology-11-01637-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/36e2fe0006c3/biology-11-01637-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/2e86f24adb61/biology-11-01637-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/783e5fa8e9ba/biology-11-01637-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f91/9687456/0102527b295b/biology-11-01637-g008.jpg

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The dynamics of FTO binding and demethylation from the mA motifs.mA 基序中 FTO 的结合和解甲基化动力学。
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