Wang Zhaobin, Yi Zhenfeng, Wang Qiye, Yin Lanmei, Li Jun, Xie Junyan, Yang Huansheng, Yin Yulong
National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Animals (Basel). 2022 Nov 3;12(21):3018. doi: 10.3390/ani12213018.
Niacin plays an important role in regulating the gut health of weaned piglets. In this study, 48 25-day-old weaned piglets (7.9 ± 0.20 kg) produced by 14 sows (3 to 4 piglets per sow) were randomly divided into 4 groups with 6 replicates in each group and 2 piglets in each replicate. Each group was fed diets supplemented with 22.5 (N1), 30 (N2), 45 (N3), and 75 (N4) mg/kg of niacin, respectively. Samples were taken at 7 and 14 d, respectively. The study shows that changes in niacin levels significantly affected the content of IgG and IgM in the serum (p < 0.05). Niacin had a significant effect on antioxidant parameters such as MDA, T-SOD, and CuZn-SOD in the jejunal mucosa of weaned piglets (p < 0.05). Moreover, significant differences were observed in the expression of cytokines such as TGF-β, TNF-α, and COX2 in the jejunal mucosa (p < 0.05). The 16S rRNA sequencing analysis showed that there were significant differences in the colonic species composition, which were also accompanied by changes in the isovaleric acid content (p < 0.05). In conclusion, an appropriate increase in niacin dose based on NRC (2012) has an important role in improving the antioxidant status of weaned piglets, alleviating intestinal inflammation in piglets, improving immunity, and regulating the structure of the microbiota.
烟酸在调节断奶仔猪肠道健康方面发挥着重要作用。在本研究中，将14头母猪所产的48头25日龄断奶仔猪（7.9±0.20千克，每头母猪产3至4头仔猪）随机分为4组，每组6个重复，每个重复2头仔猪。每组分别饲喂添加22.5（N1）、30（N2）、45（N3）和75（N4）毫克/千克烟酸的日粮。分别在第7天和第14天采集样本。研究表明，烟酸水平的变化显著影响血清中IgG和IgM的含量（p<0.05）。烟酸对断奶仔猪空肠黏膜中的抗氧化参数如丙二醛（MDA）、总超氧化物歧化酶（T-SOD）和铜锌超氧化物歧化酶（CuZn-SOD）有显著影响（p<0.05）。此外，在空肠黏膜中细胞因子如转化生长因子-β（TGF-β）、肿瘤坏死因子-α（TNF-α）和环氧化酶2（COX2）的表达上观察到显著差异（p<0.05）。16S rRNA测序分析表明，结肠菌群组成存在显著差异，同时异戊酸含量也发生了变化（p<0.05）。总之，在NRC（2012）基础上适当增加烟酸剂量对提高断奶仔猪抗氧化状态、减轻仔猪肠道炎症、提高免疫力以及调节微生物群结构具有重要作用。
