生物合成的氧化铜纳米颗粒对……具有高度抗真菌活性。

Highly Antifungal Activity of Biosynthesized Copper Oxide Nanoparticles against .

作者信息

Garcia-Marin Luis Enrique, Juarez-Moreno Karla, Vilchis-Nestor Alfredo Rafael, Castro-Longoria Ernestina

机构信息

Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Carr. Tijuana-Ensenada 3918, Zona Playitas, Ensenada 22860, Baja California, Mexico.

Center for Applied Physics and Advanced Technology, UNAM, Blvd. Juriquilla 3001, Juriquilla La Mesa, Juriquilla 76230, Queretaro, Mexico.

出版信息

Nanomaterials (Basel). 2022 Nov 1;12(21):3856. doi: 10.3390/nano12213856.

(ATCC SC5314) was exposed to biosynthesized copper oxide nanoparticles (CuONPs) to determine their inhibitory capacity. Nanoparticles were polydisperse of small size (5.8 ± 3.5 nm) with irregular shape. The minimum inhibitory concentration (MIC) against was 35.5 µg/mL. The production of reactive oxygen species (ROS) of was verified when exposed to different concentrations of CuONPs. Ultrastructural analysis of revealed a high concentration of CuONPs in the cytoplasm and outside the cell; also, nanoparticles were detected within the cell wall. Cytotoxic analyses using fibroblasts (L929), macrophages (RAW 264.7), and breast (MCF-12) cell lines show good results of cell viability when exposed at the MIC. Additionally, a hemocompatibility analysis was carried out and was found to be below 5%, considered the threshold for biocompatibility. Therefore, it is concluded that the biosynthesized CuONPs have a high potential for developing a topical antifungal treatment.

（ATCC SC5314）暴露于生物合成的氧化铜纳米颗粒（CuONPs）中以确定其抑制能力。纳米颗粒多分散，尺寸小（5.8±3.5纳米），形状不规则。对其的最低抑菌浓度（MIC）为35.5微克/毫升。当暴露于不同浓度的CuONPs时，其活性氧（ROS）的产生得到了验证。对其的超微结构分析显示，细胞质和细胞外有高浓度的CuONPs；此外，在细胞壁内也检测到了纳米颗粒。使用成纤维细胞（L929）、巨噬细胞（RAW 264.7）和乳腺（MCF-12）细胞系进行的细胞毒性分析表明，在MIC浓度下暴露时细胞活力有良好结果。此外，还进行了血液相容性分析，发现其低于5%，这被认为是生物相容性的阈值。因此，得出结论，生物合成的CuONPs在开发局部抗真菌治疗方面具有很高的潜力。