Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, China.
A Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, and with the School of Engineering Medicine, Beihang University, Beijing, 100083, China.
Biosens Bioelectron. 2023 Feb 1;221:114908. doi: 10.1016/j.bios.2022.114908. Epub 2022 Nov 12.
Glucose detection is vital in the food industry for safety and quality management. As a healthy ingredient, the flavor of honey is frequently impacted by the crystallization of glucose. Therefore, determining the glucose level can offer precise reference data for the manufacture of honey. Various approaches have been tried, and the enzyme-based electrochemical analytical method is one of the most important and widely used strategies. However, there are still challenges for most electrochemical methods to achieve stable detection resistant to temperature variation due to the easy inactivation of the enzyme, the poor anti-interference capacity of the detection techniques and other influences from the external environment. Herein, a hydrogel-based electrochemical biosensor is proposed to stably detect glucose even at wide ranges of temperatures via electrochemical impedance spectroscopic (EIS) measurement. The key factor for stable detection relies on the metal-organic framework nanoparticles' protective layer to guarantee the robustness of glucose oxidase (GOx), thereby achieving stable and specific detection for glucose. Moreover, a cascade reaction-induced hydrogel formation in a 3D structure can be used as an impedance readout, which not only amplifies but also further stabilizes the GOx-induced response. The prepared hydrogel-based electrochemical biosensor showed a linear response to the glucose concentration in the range of 0.75-4 mg/mL. Furthermore, the biosensor has excellent anti-interference and temperature stability. High performance liquid chromatography analysis also validated the accuracy of this biosensor in detecting glucose in the honey sample.
葡萄糖检测在食品工业中对于安全和质量管理至关重要。作为一种健康的成分，蜂蜜的味道经常受到葡萄糖结晶的影响。因此，确定葡萄糖水平可以为蜂蜜的制造提供精确的参考数据。已经尝试了各种方法，基于酶的电化学生物分析方法是最重要和最广泛使用的策略之一。然而，由于酶易失活、检测技术的抗干扰能力差以及外部环境等其他因素的影响，大多数电化学生物传感器方法在实现稳定检测方面仍然存在挑战，无法抵抗温度变化。本文提出了一种基于水凝胶的电化学生物传感器，通过电化学阻抗谱（EIS）测量，可以稳定地检测葡萄糖，即使在宽温度范围内也能如此。稳定检测的关键因素依赖于金属-有机骨架纳米粒子的保护层，以保证葡萄糖氧化酶（GOx）的坚固性，从而实现对葡萄糖的稳定和特异性检测。此外，3D 结构中级联反应诱导的水凝胶形成可以用作阻抗读出，不仅可以放大，而且可以进一步稳定 GOx 诱导的响应。所制备的基于水凝胶的电化学生物传感器对 0.75-4mg/mL 范围内的葡萄糖浓度表现出线性响应。此外，该生物传感器具有出色的抗干扰性和温度稳定性。高效液相色谱分析也验证了该生物传感器在检测蜂蜜样品中葡萄糖的准确性。
