Electrochemical Methods Laboratory-Analytical and Applied Chemistry Department, ETS Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta, 390, 08017 Barcelona, Spain.
Sensors (Basel). 2017 Nov 14;17(11):2620. doi: 10.3390/s17112620.
Amperometric biosensors based on the use of glucose oxidase (GOx) are able to combine the robustness of electrochemical techniques with the specificity of biological recognition processes. However, very little information can be found in literature about the fundamental analytical parameters of these sensors. In this work, the analytical behavior of an amperometric biosensor based on the immobilization of GOx using a hydrogel (Chitosan) onto highly ordered titanium dioxide nanotube arrays (TiO₂NTAs) has been evaluated. The GOx-Chitosan/TiO₂NTAs biosensor showed a sensitivity of 5.46 μA·mM with a linear range from 0.3 to 1.5 mM; its fundamental analytical parameters were studied using a commercial soft drink. The obtained results proved sufficient repeatability (RSD = 1.9%), reproducibility (RSD = 2.5%), accuracy (95-105% recovery), and robustness (RSD = 3.3%). Furthermore, no significant interferences from fructose, ascorbic acid and citric acid were obtained. In addition, the storage stability was further examined, after 30 days, the GOx-Chitosan/TiO₂NTAs biosensor retained 85% of its initial current response. Finally, the glucose content of different food samples was measured using the biosensor and compared with the respective HPLC value. In the worst scenario, a deviation smaller than 10% was obtained among the 20 samples evaluated.
基于葡萄糖氧化酶（GOx）使用的电流型生物传感器能够将电化学技术的稳健性与生物识别过程的特异性相结合。然而，关于这些传感器的基本分析参数，文献中几乎没有相关信息。在这项工作中，评估了基于将 GOx 固定在高度有序的二氧化钛纳米管阵列（TiO₂NTAs）上的水凝胶（壳聚糖）上的电流型生物传感器的分析行为。GOx-Chitosan/TiO₂NTAs 生物传感器的灵敏度为 5.46 μA·mM，线性范围为 0.3 至 1.5 mM；使用商业软饮料研究了其基本分析参数。获得的结果证明了足够的重复性（RSD = 1.9%）、重现性（RSD = 2.5%）、准确性（回收率为 95-105%）和稳健性（RSD = 3.3%）。此外，没有检测到来自果糖、抗坏血酸和柠檬酸的显著干扰。此外，还进一步检查了储存稳定性，经过 30 天后，GOx-Chitosan/TiO₂NTAs 生物传感器保留了其初始电流响应的 85%。最后，使用生物传感器测量了不同食品样品的葡萄糖含量，并与相应的 HPLC 值进行了比较。在评估的 20 个样本中，最差情况下的偏差小于 10%。
