Arczewska-Włosek Anna, Świątkiewicz Sylwester, Ognik Katarzyna, Józefiak Damian
Department of Animal Nutrition and Feed Science, National Research Institute of Animal Production, 32-083 Balice, Poland.
Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, 20-950 Lublin, Poland.
Animals (Basel). 2022 Dec 10;12(24):3489. doi: 10.3390/ani12243489.
A total of 256 male Ross 308 chickens were assigned to four treatments in a 2 × 2 factorial design with two levels of the anticoccidial vaccine (ACV) Livacox T (none or 1 × dose) with or without dietary supplementation with the probiotic Protexin (P). The growth performance parameters for the test periods (1-21, 22-42, and 1-42 d) and oocyst per gram (OPG) at weekly intervals were analysed. Blood samples were collected at 16 post-vaccination (pv) days to measure selected haematological, biochemical, redox, and immunological parameters. ACV administration worsened the performance parameters of the chickens for 1-21 d pv, while supplementation with P reduced this negative effect with a significant improvement in 1-21 d body weight gain and feed conversion ratio. ACV administration increased % phagocytic cells (%PC), phagocytic index (PI), respiratory burst activity, proportion of monocytes, and activities of aspartate aminotransferase (AST) and lactate dehydrogenase, while it decreased the catalase activity and concentration of malondialdehyde and peroxides. The dietary administration of P significantly increased counts of red blood cells and white blood cells and increased %PC and PI, while it decreased the heterophil proportion, heterophil/lymphocyte ratio ( = 0.059), and alanine aminotransferase and AST activities. The oocyst counts were comparable in all sampling periods, except on 14 d pv, as supplementation with P significantly decreased 14 d OPG, thus indicating a positive influence of P on immunity development. In conclusion, dietary supplementation with P led to improved performance, better immunity, and benefits in health status in broilers vaccinated with the ACV, without interfering with the circulating vaccine strains.
总共256只雄性罗斯308肉鸡被分配到一个2×2析因设计的四种处理中，该设计有两个抗球虫疫苗（ACV）Livacox T水平（无或1倍剂量），分别添加或不添加益生菌Protexin（P）。分析了试验期（1 - 21、22 - 42和1 - 42天）的生长性能参数以及每周每克粪便中的卵囊数（OPG）。在接种疫苗后16天采集血样，以测量选定的血液学、生化、氧化还原和免疫学参数。接种ACV在接种后1 - 21天降低了肉鸡的性能参数，而添加P减轻了这种负面影响，显著提高了1 - 21天的体重增加和饲料转化率。接种ACV增加了吞噬细胞百分比（%PC）、吞噬指数（PI）、呼吸爆发活性、单核细胞比例以及天冬氨酸转氨酶（AST）和乳酸脱氢酶的活性，同时降低了过氧化氢酶活性以及丙二醛和过氧化物的浓度。日粮添加P显著增加了红细胞和白细胞计数，提高了%PC和PI，同时降低了嗜异性粒细胞比例、嗜异性粒细胞/淋巴细胞比率（=0.059）以及丙氨酸转氨酶和AST的活性。除接种后14天外，所有采样期的卵囊计数相当，因为添加P显著降低了接种后14天的OPG，这表明P对免疫发育有积极影响。总之，日粮添加P可提高接种ACV的肉鸡的生产性能、增强免疫力并改善健康状况，且不干扰循环疫苗株。
