Wang Fang, Zhufeng Yunzhi, Chen Zhe, Xu Jun, Cheng Yongjing
Department of Rheumatology and Immunology Beijing Hospital, National Centre of Gerontology, Beijing, China.
Department of Rheumatology, Peking University People's Hospital, Beijing, China.
Clin Rheumatol. 2023 May;42(5):1315-1326. doi: 10.1007/s10067-022-06451-1. Epub 2023 Jan 4.
This healthy volunteer control-based study was conducted to explore alterations of compositions and function of gut microbiota in Chinese pSS patients.
The high-throughput Illumina Miseq sequencing method, targeting the V3-V4 region of the 16S ribosomal RNA (rRNA) gene, was used to compare the microbiota communities between 30 pSS patients and 30 age-matched healthy volunteers. The intestinal dysbiosis of pSS patients was evaluated and its correlation with some disease phenotypes was analyzed. Furthermore, we performed the amino acid sequence alignment analysis to illustrate the molecular mimicry patterns of new microbial peptides.
Compared with that in healthy controls, the composition and function of the gut microbiota significantly differed in pSS patients. Certain genera and species, including genera: Escherichia-Shigella, Sardovia, Veillonella, Insteinimonas, and Lactobacillales; species: Escherichia coli, Lactobacillus phage Sal3, Lactobacillus reuteri, Lactobacillus gasseri, Streptococcus lutetiensis, Streptococcus mutans, Scardovia wiggsiae, and Fusobacterrium ulcerans were found to be enriched in the feces of pSS patients, while butyrate-producing bacteria were less abundant in pSS patients. Certain genera (including Lactobacillales) and species (including Lactobacillus gasseri) were associated with disease severity and therapy resistance parameters. Autoantigen epitopes of "WPSALPT, NPARSFG, MNPARSFG, and AFGLAIGT" from aquaporin-5 were aligned perfectly with one enriched microbiota of patients with pSS, namely Escherichia coli.
The composition and function of the gut microbiota significantly differed in pSS patients compared with that in healthy controls. Our study would facilitate the possible research on the role of gut microbiota in the pathogenesis of pSS.
本研究以健康志愿者为对照，旨在探索中国原发性干燥综合征（pSS）患者肠道微生物群的组成和功能变化。
采用针对16S核糖体RNA（rRNA）基因V3-V4区域的高通量Illumina Miseq测序方法，比较30例pSS患者和30例年龄匹配的健康志愿者的微生物群落。评估pSS患者的肠道菌群失调情况，并分析其与某些疾病表型的相关性。此外，我们进行了氨基酸序列比对分析，以阐明新微生物肽的分子模拟模式。
与健康对照相比，pSS患者肠道微生物群的组成和功能存在显著差异。在pSS患者粪便中发现某些属和种富集，包括埃希氏菌属-志贺氏菌属、萨多维亚菌属、韦荣球菌属、因斯坦氏菌属和乳杆菌目；种包括大肠杆菌、嗜酸乳杆菌噬菌体Sal3、罗伊氏乳杆菌、加氏乳杆菌、路氏链球菌、变形链球菌、威氏斯卡德氏菌和溃疡梭杆菌，而pSS患者中产生丁酸盐的细菌较少。某些属（包括乳杆菌目）和种（包括加氏乳杆菌）与疾病严重程度和治疗抵抗参数相关。水通道蛋白-5的“WPSALPT、NPARSFG、MNPARSFG和AFGLAIGT”自身抗原表位与pSS患者一种富集的微生物群，即大肠杆菌，完美比对。
与健康对照相比，pSS患者肠道微生物群的组成和功能存在显著差异。我们的研究将有助于对肠道微生物群在pSS发病机制中的作用进行可能的研究。
