Tokranova N, Cady N, Lampher A, Levitsky I A
SUNY Polytechnic Institute, Albany NY 12203.
Emitech, Inc. Fall River, MA 02720.
IEEE Sens J. 2022 Nov 1;22(21):20165-20170. doi: 10.1109/jsen.2022.3200591. Epub 2022 Aug 26.
Rapid and accurate detection of fentanyl (highly potent opioid) is a critical importance due to current opioids crisis worldwide. We report the highly sensitive detection of fentanyl utilizing the synergetic effect of nanoporous silicon as a substrate with a high interfacial area and specific antibody functionalization of nanoporous silicon. The electrochemical sensor consists of gold working and counter electrodes deposited on nanoprous silicon, antibodies immobilized between these electrodes and an Ag/AgCl reference electrode. Square wave voltammetry was used as an electrochemical transduction method. The detection limit was determined as 6 ng/ml and 11.5 ng/ml for specific peak (fentanyl signature) in phosphate buffer and human sweat, respectively. A future goal of this study is to a wearable sweat sensor array for rapid and on-site detection of multiple opioids with analytical sensitivity comparable with laboratory tests.
由于当前全球阿片类药物危机，快速准确地检测芬太尼（高效阿片类药物）至关重要。我们报告了利用纳米多孔硅作为具有高界面面积的底物与纳米多孔硅的特异性抗体功能化的协同效应来高度灵敏地检测芬太尼。该电化学传感器由沉积在纳米多孔硅上的金工作电极和对电极、固定在这些电极之间的抗体以及Ag/AgCl参比电极组成。采用方波伏安法作为电化学转换方法。在磷酸盐缓冲液和人汗液中，特定峰（芬太尼特征峰）的检测限分别确定为6 ng/ml和11.5 ng/ml。本研究的一个未来目标是开发一种可穿戴汗液传感器阵列，用于快速现场检测多种阿片类药物，其分析灵敏度与实验室测试相当。
