Sun Wenbo, Wang Zonghua, Liu Jianhua, Jiang Chunhuan, Chen Weihua, Yu Bin, Wang Wei, Lu Lehui
College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao 266071, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao 266071, China.
Sci Bull (Beijing). 2021 Apr 15;66(7):676-684. doi: 10.1016/j.scib.2020.10.020. Epub 2020 Oct 31.
Theranostic nanoprobes can potentially integrate imaging and therapeutic capabilities into a single platform, offering a new personalized cancer diagnostic tool. However, there is a growing concern that their clinical application is not safe, particularly due to metal-containing elements, such as the gadolinium used in magnetic resonance imaging (MRI). We demonstrate for the first time that the photothermal melting of the DNA duplex helix was a reliable and versatile strategy that enables the on-demand degradation of the gadolinium-containing MRI reporter gene from polydopamine (PDA)-based theranostic nanoprobes. The combination of chemotherapy (doxorubicin) and photothermal therapy, which leads to the enhanced anti-tumor effect. In vivo MRI tracking reveals that renal filtration was able to rapidly clear the free gadolinium-containing MRI reporter from the mice body. This results in a decrease in the long-term toxic effect of theranostic MRI nanoprobes. Our findings may pave the way to address toxicity issues of the theranostic nanoprobes.
诊疗纳米探针有可能将成像和治疗能力整合到一个单一平台上，提供一种新型的个性化癌症诊断工具。然而，人们越来越担心它们的临床应用不安全，特别是由于含有金属的元素，如磁共振成像（MRI）中使用的钆。我们首次证明，DNA双链螺旋的光热熔化是一种可靠且通用的策略，能够从基于聚多巴胺（PDA）的诊疗纳米探针中按需降解含钆的MRI报告基因。化疗（阿霉素）和光热疗法相结合，可增强抗肿瘤效果。体内MRI跟踪显示，肾脏过滤能够迅速从小鼠体内清除游离的含钆MRI报告基因。这导致诊疗MRI纳米探针的长期毒性作用降低。我们的发现可能为解决诊疗纳米探针的毒性问题铺平道路。
