Winchester R J, Wang C Y, Gibofsky A, Kunkel H G, Lloyd K O, Old L J
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6235-9. doi: 10.1073/pnas.75.12.6235.
Human malignant melanoma cell lines were found to bear Ia-like cell surface determinants demonstrable by hetero- or alloantisera and by direct identification of the characteristic bimolecular glycoprotein complex. Immunoprecipitation confirmed the presence of Ia determinants on the bimolecular complex. The quantity of Ia molecules determined by these methods and by absorption experiments was relatively constant for each cell line. Among different lines, however, the amount of Ia antigens ranged from a level equal to that expressed by B-cell lines to a small fraction of this amount. This variation in level of Ia contrasted with the more uniform amount of beta2-microglobulin detected on the cell surface. The Ia alloantigen specificity DRw2 was the most frequently encountered specificity. Ia determinants were also found on the surface of an epidermoid carcinoma line, but not on various other cell lines of normal or neoplastic origin.
人类恶性黑色素瘤细胞系被发现带有可通过异种或同种抗血清以及通过直接鉴定特征性双分子糖蛋白复合物而证实的Ia样细胞表面决定簇。免疫沉淀证实双分子复合物上存在Ia决定簇。通过这些方法以及吸收实验测定的Ia分子数量对于每个细胞系而言相对恒定。然而，在不同的细胞系中，Ia抗原的量从与B细胞系表达的水平相当到该量的一小部分不等。Ia水平的这种变化与在细胞表面检测到的β2-微球蛋白量更为一致形成对比。Ia同种异体抗原特异性DRw2是最常遇到的特异性。在一个表皮样癌细胞系的表面也发现了Ia决定簇，但在正常或肿瘤来源的各种其他细胞系上未发现。
