Persson B David, Schmidt Jacob Günther, Hakhverdyan Mikhayil, Leijon Mikael, Olesen Niels Jørgen, Axén Charlotte
National Veterinary Institute, 751 89 Uppsala, Sweden.
National Institute of Aquatic Resources, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
Vet Sci. 2023 Jan 14;10(1):58. doi: 10.3390/vetsci10010058.
In November 2016, infectious pancreatic necrosis virus (IPNV) was isolated from a broodstock female of landlocked sea trout () in Lake Vänern in Sweden. VP2 gene sequencing placed the IPNV isolate in genogroup 6, for which pathogenicity is largely unknown. Lake Vänern hosts landlocked sea trout and salmon populations that are endangered, and thus the introduction of new pathogens poses a major threat. In this study we characterized the novel isolate by conducting an infection trial on three salmonid species present in Lake Vänern, whole genome sequencing of the isolate, and prevalence studies in the wild sea trout and salmon in Lake Vänern. During the infection trial, the pathogenicity of the Swedish isolate was compared to that of a pathogenic genogroup 5 isolate. Dead or moribund fish were collected, pooled, and analyzed by cell culture to identify infected individuals. In the trial, the Swedish isolate was detected in fewer sample pools in all three species compared to the genogroup 5 isolate. In addition, the prevalence studies showed a low prevalence (0.2-0.5%) of the virus in the feral salmonids in Lake Vänern. Together the data suggest that the novel Swedish IPNV genogroup 6 isolate is only mildly pathogenic to salmonids.
2016年11月，在瑞典韦纳恩湖的一条内陆海鳟（）亲鱼雌鱼体内分离出传染性胰腺坏死病毒（IPNV）。VP2基因测序将该IPNV分离株归为基因组6型，其致病性在很大程度上未知。韦纳恩湖中有濒临灭绝的内陆海鳟和鲑鱼种群，因此新病原体的引入构成了重大威胁。在本研究中，我们通过对韦纳恩湖中存在的三种鲑科鱼类进行感染试验、对该分离株进行全基因组测序以及在韦纳恩湖野生海鳟和鲑鱼中进行患病率研究，对该新型分离株进行了特征描述。在感染试验期间，将瑞典分离株的致病性与基因组5型致病分离株的致病性进行了比较。收集死亡或濒死的鱼，合并后通过细胞培养进行分析，以确定受感染的个体。在试验中，与基因组5型分离株相比，在所有三个物种的较少样本组中检测到了瑞典分离株。此外，患病率研究表明，韦纳恩湖野生鲑科鱼类中该病毒的患病率较低（0.2 - 0.5%）。综合这些数据表明，新型瑞典IPNV基因组6型分离株对鲑科鱼类仅具有轻度致病性。
