Centre for Drug Research, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Molecules. 2011 Apr 8;16(4):3037-47. doi: 10.3390/molecules16043037.
This study was designed to investigate the antimicrobial activity of Cinnamomum iners standardized leave methanolic extract (CSLE), its fractions and isolated compounds. CSLE and fractions were subjected to disc diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests using different Gram positive and Gram negative bacteria and yeast. Within the series of fractions tested, the ethyl acetate fraction was the most active, particularly against methicillin resistant Staphylococcus aureus (MRSA) and Escherichia coli, with MIC values of 100 and 200 µg/mL, respectively. The active compound in this fraction was isolated and identified as xanthorrhizol [5-(1, 5-dimethyl-4-hexenyl)-2-methylphenol] by various spectroscopic techniques. The overall results of this study provide evidence that Cinnamomum iners leaves extract as well as the isolated compound xanthorrhizol exhibit antimicrobial activity for both Gram negative and Gram positive pathogens, especially against MRSA strains.
本研究旨在探究肉桂树不规则叶甲醇提取物（CSLE）及其馏分和分离化合物的抗菌活性。采用不同的革兰氏阳性菌和革兰氏阴性菌及酵母，对 CSLE 和馏分进行了圆盘扩散、最小抑菌浓度（MIC）和最小杀菌浓度（MBC）试验。在所测试的馏分系列中，乙酸乙酯馏分活性最强，特别是对耐甲氧西林金黄色葡萄球菌（MRSA）和大肠杆菌，MIC 值分别为 100 和 200µg/mL。该馏分中的活性化合物通过各种光谱技术被分离并鉴定为黄樟素[5-(1,5-二甲基-4-己烯基)-2-甲基苯酚]。本研究的总体结果表明，肉桂不规则叶提取物以及分离出的化合物黄樟素对革兰氏阴性和革兰氏阳性病原体均具有抗菌活性，特别是对 MRSA 菌株。
