Butdee Waranya, Muangham Supattra, Chonudomkul Duenrut, Duangmal Kannika
Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005639.
The taxonomic position of a novel actinomycete, designated strain RS10V-4, was determined using a polyphasic approach. Strain RS10V-4 was isolated from paddy rhizosphere soil of rice plant ( L.). The morphological, physiological and chemotaxonomic properties were consistent with its classification in the genus . On the basis of 16S rRNA gene sequence analysis, strain RS10V-4 belongs to the genus and had the highest sequence similarity to NBRC 15452 (98.3 %). The G+C content of the genomic DNA was 73.8 %. Digital DNA-DNA hybridization and average nucleotide identity values between the genome sequences of strain RS10V-4 and ATCC 11455 were lower than the recommendation threshold values for the recognition of species within the same genus. The whole-cell hydrolysates of strain RS10V-4 contained diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were glucose and ribose. The predominant menaquinones were MK-9(H) and MK-9(H). The predominant cellular fatty acids (>10 %) were iso-C, anteiso-C, iso-C and iso-C. The polar lipids of strain RS10V-4 contained diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminolipid, two unidentified lipids and an unidentified phospholipid. On the basis of these phenotypic and genotypic characteristics, it is supported that strain RS10V-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RS10V-4 (=TBRC 15167=NBRC 115345). In addition, the comparison of the whole genome sequences and phenotypic features suggested that and belong to the same species. Therefore, it is proposed that is reclassified as a later heterotypic synonym of .
采用多相分类法确定了一株新的放线菌（命名为RS10V-4菌株）的分类地位。RS10V-4菌株从水稻（L.）的稻田根际土壤中分离得到。其形态、生理和化学分类学特性与其在该属中的分类一致。基于16S rRNA基因序列分析，RS10V-4菌株属于该属，与NBRC 15452的序列相似性最高（98.3%）。基因组DNA的G+C含量为73.8%。RS10V-4菌株与ATCC 11455的基因组序列之间的数字DNA-DNA杂交和平均核苷酸同一性值低于同一属内物种识别的推荐阈值。RS10V-4菌株的全细胞水解产物含有二氨基庚二酸作为诊断性二氨基酸，全细胞糖类为葡萄糖和核糖。主要的甲基萘醌是MK-9(H)和MK-9(H)。主要的细胞脂肪酸（>10%）是异-C、anteiso-C、异-C和异-C。RS10V-4菌株的极性脂质包含双磷脂酰甘油、羟基磷脂酰乙醇胺、磷脂酰乙醇胺、磷脂酰肌醇、一种未鉴定的氨基脂质、两种未鉴定的脂质和一种未鉴定的磷脂。基于这些表型和基因型特征，支持RS10V-4菌株代表该属的一个新物种，为此提出新物种名为sp. nov.。模式菌株为RS10V-4（=TBRC 15167=NBRC 115345）。此外，全基因组序列和表型特征的比较表明和属于同一物种。因此，建议将重新分类为的晚出异名。
