Yang Chunhua, Zhu Jierui, Bai Jing, Zhang Jie, Wu Zhihua, Li Xin, Tong Ping, Chen Hongbing, Yang Anshu
State Key Laboratory of Food Science and Technology, Nanchang University, Nanjing Dong Lu 235, Nanchang 330047, China.
Sino-German Joint Research Institute, Nanchang University, Nanjing Dong Lu 235, Nanchang 330047, China.
Foods. 2023 Feb 1;12(3):627. doi: 10.3390/foods12030627.
In the early stage of this study, three strains of with anti-soybean allergy potential were screened: CICC 6081, subsp. CICC 6103 and subsp. CICC 20988. The aim of this study was to analyze the desensitization effect of three strains of administered by gavage to soybean-allergic mice through the differentiation of immune cells in intestinal lymph nodes and the changes to gut microbiota. The results showed that the three strains of could stimulate the proliferation of dendritic cells (DCs) and regulate the balance of Th1/Th2 differentiation in the MLNs and PPs of soybean-allergic mice. Furthermore, the Th17/Tregs cell-differentiation ratio in the MLNs of the -treated mice was significantly lower than that of the allergic mice ( < 0.05). Compared to the control group, the Shannon, Sobs and Ace indexes of intestinal microbiota in the allergic mice were significantly increased ( < 0.05), and the proportion of was significantly higher ( < 0.05), which was reversed by gavage. In conclusion, the three strains of can inhibit the intestinal mucosal immune response and regulate gut microbiota balance in soybean-allergic mice.
在本研究的早期阶段，筛选出了三株具有抗大豆过敏潜力的菌株：嗜热栖热放线菌CICC 6081、嗜热栖热放线菌亚种CICC 6103和嗜热栖热放线菌亚种CICC 20988。本研究的目的是通过分析肠道淋巴结中免疫细胞的分化以及肠道微生物群的变化，来研究经灌胃给予三株嗜热栖热放线菌对大豆过敏小鼠的脱敏效果。结果表明，这三株嗜热栖热放线菌能够刺激树突状细胞（DCs）的增殖，并调节大豆过敏小鼠肠系膜淋巴结（MLNs）和派尔集合淋巴结（PPs）中Th1/Th2分化的平衡。此外，经嗜热栖热放线菌处理的小鼠肠系膜淋巴结中Th17/Tregs细胞分化比率显著低于过敏小鼠（P<0.05）。与对照组相比，过敏小鼠肠道微生物群的香农指数、观测物种数和ACE指数显著升高（P<0.05），且拟杆菌属的比例显著更高（P<0.05），而经嗜热栖热放线菌灌胃后这些情况得到了逆转。总之，这三株嗜热栖热放线菌可以抑制大豆过敏小鼠的肠道黏膜免疫反应并调节肠道微生物群平衡。
