Institute of Physics, Slovak Academy of Sciences, Dúbravská Cesta 9, SK-84511 Bratislava, Slovakia.
Center for Advanced Materials Application, Slovak Academy of Sciences, Dúbravská Cesta 9, SK-84511 Bratislava, Slovakia.
Molecules. 2023 Jan 19;28(3):1018. doi: 10.3390/molecules28031018.
The biocolonization of building materials by microorganisms is one of the main causes of their degradation. Fungi and bacteria products can have an undesirable impact on human health. The protection and disinfection of sandstone and wood materials are of great interest. In this study, we evaluated the protection and disinfection activity of oregano and thyme essential oils encapsulated in poly(ε-caprolactone) nanocapsules (Or-NCs, Th-NCs) against four types of environmental microorganisms: , (fungal strains), and sp. (bacterial strains). The surfaces of sandstone and whitewood samples were inoculated with these microorganisms before or after applying Or-NCs and Th-NCs. The concentration-dependent effect of Or-NCs and Th-NCs on biofilm viability was determined by the MTT reduction assay. The results showed that Or-NCs and Th-NCs possess effective disinfection and anti-biofilm activity. Diffuse reflectivity measurements revealed no visible color changes of the materials after the application of the nanoencapsulated essential oils.
微生物对建筑材料的生物殖民化是导致其降解的主要原因之一。真菌和细菌产物可能对人类健康产生不良影响。因此，对砂岩和木材材料的保护和消毒具有重要意义。在这项研究中，我们评估了封装在聚己内酯纳米胶囊（Or-NCs、Th-NCs）中的牛至和百里香精油的保护和消毒活性，针对四种类型的环境微生物： 、 （真菌菌株）和 sp.（细菌菌株）。在将 Or-NCs 和 Th-NCs 施加到砂岩和白木样品表面之前或之后，用这些微生物对其进行接种。通过 MTT 还原测定法确定了 Or-NCs 和 Th-NCs 对生物膜活力的浓度依赖性影响。结果表明，Or-NCs 和 Th-NCs 具有有效的消毒和抗生物膜活性。漫反射测量显示，纳米封装精油施加后材料没有可见的颜色变化。
