Ahlering T E, Dubeau L, Jones P A
Urological Cancer Research Laboratory, USC Comprehensive Cancer Center, Los Angeles 90033.
Cancer Res. 1987 Dec 15;47(24 Pt 1):6660-5.
An animal model to investigate the invasive and metastatic properties of human bladder transitional cell carcinoma (HTCC) was established. Two long-term HTCC cell lines (RT4 and EJ) and one HTCC cell line derived in our laboratory (LD-71) were tumorigenic when injected s.c. into nude mice but had little potential to invade locally or metastasize before the animals succumbed to tumor burden. Experimental lung metastases were, however, observed in approximately 60% of animals given injections of RT4 or EJ cell lines in the tail vein. The cells were also implanted transurethrally into the urinary bladders of athymic mice. RT4 cells, which were originally isolated from a superficial papillary tumor, produced histologically noninvasive tumors after transurethral inoculation with no evidence of metastasis. In contrast EJ cells, which were originally isolated from a more aggressive tumor, produced invasive tumors in nude mouse bladders and metastasized to the lungs spontaneously. The invasive cell line LD-71 was weakly tumorigenic and was successfully implanted into the bladder on only one of five attempts. The human origin of the implanted tumors was documented by Southern blot analysis using human repetitive DNA as a probe. The results indicate that the site of injection strongly influences the behavior of the resulting tumors and that intravesical implantation of these HTCC cell lines produces pathological expression of invasiveness and metastatic potential.
建立了一种用于研究人膀胱移行细胞癌（HTCC）侵袭和转移特性的动物模型。两种长期培养的HTCC细胞系（RT4和EJ）以及我们实验室衍生的一种HTCC细胞系（LD - 71），皮下注射到裸鼠体内时具有致瘤性，但在动物死于肿瘤负荷之前，它们局部侵袭或转移的潜力很小。然而，在大约60%经尾静脉注射RT4或EJ细胞系的动物中观察到了实验性肺转移。这些细胞也经尿道植入无胸腺小鼠的膀胱。RT4细胞最初从浅表乳头状肿瘤中分离出来，经尿道接种后产生组织学上无侵袭性的肿瘤，且无转移迹象。相比之下，EJ细胞最初从侵袭性更强的肿瘤中分离出来，在裸鼠膀胱中产生侵袭性肿瘤并自发转移至肺部。侵袭性细胞系LD - 71致瘤性较弱，在五次尝试中只有一次成功植入膀胱。使用人类重复DNA作为探针，通过Southern印迹分析证实了植入肿瘤的人类起源。结果表明，注射部位对所产生肿瘤的行为有强烈影响，并且这些HTCC细胞系经膀胱内植入会产生侵袭性和转移潜能的病理表现。
