Department of Central Laboratory, Jintan Hospital, Jiangsu University, Jintan, China.
Jiangsu Key Laboratory of Clinical Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
FEBS J. 2023 Jul;290(13):3359-3368. doi: 10.1111/febs.16756. Epub 2023 Mar 6.
Migrasomes comprise a recently identified unique type of extracellular vesicle (EV) containing varying numbers of small vesicles. However, the final fate of these small vesicles is still unclear. Here, we report the discovery of EV-like migrasome-derived nanoparticles (MDNPs) that are produced by migrasomes releasing internal vesicles via self-rupture and through a process similar to cell plasma membrane budding. Our results demonstrate that MDNPs have a membrane structure with a typical round-shaped morphology and have the characteristic markers of migrasomes, but do not present the markers of EVs from the cell culture supernatant. More importantly, we also show that MDNPs are loaded with a large number of microRNAs different from those found in migrasomes and EVs. Our results provide evidence that migrasomes can produce EV-like nanoparticles. These findings have important implications for understanding the unknown biological functions of migrasomes.
迁移体包含一种最近被鉴定的独特的细胞外囊泡 (EV) 类型，其中含有数量不等的小囊泡。然而，这些小囊泡的最终命运仍然不清楚。在这里，我们报告了 EV 样迁移体衍生的纳米颗粒 (MDNPs) 的发现，这些纳米颗粒是由迁移体通过自身破裂和类似于细胞膜出芽的过程释放内部囊泡而产生的。我们的结果表明，MDNPs 具有膜结构，呈典型的圆形形态，具有迁移体的特征标记物，但不存在细胞培养上清液中 EV 的标记物。更重要的是，我们还表明，MDNPs 负载着大量不同于迁移体和 EV 中发现的 microRNAs。我们的结果提供了证据表明迁移体可以产生类似 EV 的纳米颗粒。这些发现对于理解迁移体未知的生物学功能具有重要意义。
