Shaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau, College of Life Sciences, Yan'an University, Yan'an 716000, China.
Engineering Research Center of Microbial Resources Development and Green Recycling of Shaanxi Province, Yan'an University, Yan'an 716000, China.
Int J Mol Sci. 2024 Sep 29;25(19):10487. doi: 10.3390/ijms251910487.
Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) plays pivotal roles in regulating plant growth and development, mediating intercellular signal transduction, and modulating responses to environmental stresses. However, a comprehensive genome-wide identification and analysis of the gene family in maize remains elusive. In this study, a total of 24 genes were identified in the maize whole genome. A phylogenetic analysis further revealed that CrRLK1L proteins from , rice, and maize were grouped into nine distinct subgroups, with subgroup IV being unique to maize. Gene structure analysis demonstrated that the number of introns varied greatly among genes. Notably, the genome-wide duplication (WGD) events promoted the expansion of the gene family. Compared with , there were more collinear gene pairs between maize and rice. Tissue expression patterns indicated that genes are widely expressed in various tissues, with specifically highly expressed in roots, and expressed in anthers. Additionally, RNA-seq and RT-qPCR analyses revealed that the expression of genes exhibited different expression patterns under drought and salt stresses. In summary, our study lays a foundation for elucidating the biological roles of genes in maize growth and development, reproductive development, and stress responses.
长春花受体样激酶 1 样（CrRLK1L）在调节植物生长发育、介导细胞间信号转导以及调节对环境胁迫的响应方面发挥着关键作用。然而，玉米中基因家族的全基因组鉴定和分析仍未得到全面了解。在这项研究中，在玉米全基因组中共鉴定出 24 个基因。系统发育分析进一步表明，长春花、水稻和玉米的 CrRLK1L 蛋白被分为九个不同的亚组，其中亚组 IV 是玉米所特有的。基因结构分析表明，基因的内含子数量差异很大。值得注意的是，全基因组复制（WGD）事件促进了基因家族的扩张。与水稻相比，玉米和水稻之间有更多的共线性基因对。组织表达模式表明，基因在各种组织中广泛表达，特别是在根中高度表达，而在花药中表达。此外，RNA-seq 和 RT-qPCR 分析表明，基因的表达在干旱和盐胁迫下表现出不同的表达模式。综上所述，我们的研究为阐明基因在玉米生长发育、生殖发育和应激响应中的生物学功能奠定了基础。
