Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Animal Resource Center, University of Texas Medical Branch, Galveston, Texas, USA.
J Infect Dis. 2023 Aug 31;228(5):604-614. doi: 10.1093/infdis/jiad053.
The common marmoset (Callithrix jacchus) is increasingly recognized as an ideal nonhuman primate (NHP) at high biocontainment due to its smaller size and relative ease of handling. Here, we evaluated the susceptibility and pathogenesis of Nipah virus Bangladesh strain (NiVB) infection in marmosets at biosafety level 4. Infection via the intranasal and intratracheal route resulted in fatal disease in all 4 infected marmosets. Three developed pulmonary edema and hemorrhage as well as multifocal hemorrhagic lymphadenopathy, while 1 recapitulated neurologic clinical manifestations and cardiomyopathy on gross pathology. Organ-specific innate and inflammatory responses were characterized by RNA sequencing in 6 different tissues from infected and control marmosets. Notably, a unique transcriptome was revealed in the brainstem of the marmoset exhibiting neurological signs. Our results provide a more comprehensive understanding of NiV pathogenesis in an accessible and novel NHP model, closely reflecting clinical disease as observed in NiV patients.
普通狨猴（Callithrix jacchus）因其体型较小且相对易于处理，正逐渐被公认为高生物安全防护水平下理想的非人类灵长类动物（NHP）。在这里，我们评估了尼帕病毒孟加拉国株（NiVB）在四级生物安全水平下感染狨猴的易感性和发病机制。通过鼻内和气管内途径感染，所有 4 只感染的狨猴均导致致命疾病。其中 3 只出现肺水肿和出血以及多灶性出血性淋巴结病，而 1 只在大体病理学上重现了神经临床症状和心肌病。通过对感染和对照狨猴的 6 种不同组织进行 RNA 测序，对其器官特异性固有和炎症反应进行了特征描述。值得注意的是，在表现出神经症状的狨猴脑干中揭示了一个独特的转录组。我们的研究结果提供了对在可及且新颖的 NHP 模型中尼帕病毒发病机制的更全面理解，与尼帕病毒患者中观察到的临床疾病非常相似。
