Guerrero-Romero Fernando, Castellanos-Juárez Francisco X, Salas-Pacheco Jose M, Morales-Gurrola Francisco G, Salas-Leal Alma Cristina, Simental-Mendía Luis E
Unidad de Investigación Biomédica, Instituto Mexicano del Seguro Social, Predio Canoas 100, Col. Los Angeles, 34077, Durango, Dgo, Mexico.
Institute of Scientific Research, Juarez University of the State of Durango, Durango, México.
Mol Biol Rep. 2023 May;50(5):4723-4728. doi: 10.1007/s11033-023-08338-z. Epub 2023 Mar 11.
Among the Toll-like receptors (TLR) that are dependent of myeloid response protein (MyD88), the TLR4 and TLR2 are directly associated with low-grade chronic inflammation; however, they are not been investigated in subjects with metabolically healthy obesity (MHO). Thus, the objective of this study was to determine the association between the expression of TLR4, TLR2, and MyD88 with low-grade chronic inflammation in individuals with MHO.
Men and women with obesity aged 20 to 55 years were enrolled in a cross-sectional study. Individuals with MHO were allocated into the groups with and without low-grade chronic inflammation. Pregnancy, smoking, alcohol consumption, intense physical activity or sexual intercourse in the previous 72 h, diabetes, high blood pressure, cancer, thyroid disease, acute or chronic infections, renal impairment, and hepatic diseases, were exclusion criteria. The MHO phenotype was defined by a body mass index (BMI ≥ 30 kg/m) plus one or none of the following cardiovascular risk factors: hyperglycemia, elevated blood pressure, hypertriglyceridemia, and low high-density lipoprotein cholesterol. A total of 64 individuals with MHO were enrolled and allocated into the groups with (n = 37) and without (n = 27) inflammation. The multiple logistic regression analysis indicated that TLR2 expression is significantly associated with inflammation in individuals with MHO. In the subsequent analysis adjusted by BMI, TLR2 expression remained associated with inflammation in individuals with MHO.
Our results suggest that overexpression of TLR2, but not TLR4 and MyD88, is associated with low-grade chronic inflammation in subjects with MHO.
在依赖髓样分化因子88（MyD88）的Toll样受体（TLR）中，TLR4和TLR2与低度慢性炎症直接相关；然而，尚未在代谢健康肥胖（MHO）受试者中对它们进行研究。因此，本研究的目的是确定TLR4、TLR2和MyD88的表达与MHO个体低度慢性炎症之间的关联。
纳入年龄在20至55岁的肥胖男性和女性进行横断面研究。将MHO个体分为有和无低度慢性炎症两组。排除标准包括妊娠、吸烟、饮酒、前72小时内剧烈体育活动或性交、糖尿病、高血压、癌症、甲状腺疾病、急性或慢性感染、肾功能损害和肝病。MHO表型由体重指数（BMI≥30kg/m）加以下一种或无心血管危险因素定义：高血糖、血压升高、高甘油三酯血症和低高密度脂蛋白胆固醇。共纳入64例MHO个体，并分为有炎症组（n = 37）和无炎症组（n = 27）。多元逻辑回归分析表明，TLR2表达与MHO个体的炎症显著相关。在随后按BMI调整的分析中，TLR2表达仍与MHO个体的炎症相关。
我们的结果表明，TLR2的过表达而非TLR4和MyD88的过表达与MHO受试者的低度慢性炎症相关。
