Jarrah Samar Afif, Kmetiuk Louise Bach, Valleriani Fabrizia, Bonfini Barbara, Lorusso Alessio, Vasinioti Violetta, Decaro Nicola, Dos Santos Marco Tulio, Spohr Kledir Anderson Hofstaetter, Pratelli Annamaria, Serroni Anna, Capista Sara, Sousa Valéria Regia Franco, Biondo Alexander Welker, Nakazato Luciano, Dutra Valéria
Laboratory of Molecular Biology, Federal University of Mato Grosso, Cuiabá, MT, Brazil.
Department of Veterinary Medicine, Federal University of Paraná, Curitiba, PR, Brazil.
Front Vet Sci. 2023 Feb 23;10:1111728. doi: 10.3389/fvets.2023.1111728. eCollection 2023.
SARS-CoV-2 was a worldwide threat during the COVID-19 pandemic, and the state of Mato Grosso had the second highest mortality rate in Brazil, with 427. 4 deaths/100,000 inhabitants. However, no large-scale study among dogs and cats in such highly infected areas of Brazil has so far been conducted. Accordingly, the present study reports on a serosurvey among dogs and cats in Cuiabá, capital of Mato Grosso from November 2020 to July 2021, where the human mortality rate was 605/100,000 at that time. Overall, 33/762 dogs (4.3%) and 4/182 cats (2.2%) were found to be seropositive for SARS-CoV-2 through ELISA, and 3/762 dogs (0.4%) and 3/182 cats (1.6%) were seropositive through the serum neutralization test. Cats presented higher seroprevalence with higher titers of neutralizing antibodies. Although N-protein based ELISA may be a good screening test, cross-reactivity with other canine coronaviruses may impair its diagnostic use among dogs.
在新冠疫情期间，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是一种全球性威胁，马托格罗索州的死亡率在巴西位居第二，为427.4例死亡/10万居民。然而，迄今为止，尚未在巴西此类高感染地区对犬猫进行大规模研究。因此，本研究报告了2020年11月至2021年7月在马托格罗索州首府库亚巴对犬猫进行的血清学调查，当时该地区的人类死亡率为605/10万。总体而言，通过酶联免疫吸附测定（ELISA）发现，762只犬中有33只（4.3%）、182只猫中有4只（2.2%）SARS-CoV-2血清呈阳性；通过血清中和试验，762只犬中有3只（0.4%）、182只猫中有3只（1.6%）血清呈阳性。猫的血清阳性率更高，中和抗体滴度也更高。尽管基于核衣壳蛋白（N蛋白）的ELISA可能是一种良好的筛查试验，但与其他犬冠状病毒的交叉反应可能会影响其在犬类中的诊断应用。
