Perez-Puyana Victor, Villanueva Paula, Jiménez-Rosado Mercedes, de la Portilla Fernando, Romero Alberto
Departamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, 41012 Sevilla, Spain.
Institute of Biomedicine of Seville (IBiS), "Virgen del Rocío" University Hospital, IBiS, CSIC/University of Seville, 41013 Sevilla, Spain.
Polymers (Basel). 2021 May 6;13(9):1501. doi: 10.3390/polym13091501.
Skeletal muscle regeneration is increasingly necessary, which is reflected in the increasing number of studies that are focused on improving the scaffolds used for such regeneration, as well as the incubation protocol. The main objective of this work was to improve the characteristics of polycaprolactone (PCL) scaffolds by incorporating elastin to achieve better cell proliferation and biocompatibility. In addition, two cell incubation protocols (with and without dynamic mechanical stimulation) were evaluated to improve the activity and functionality yields of the regenerated cells. The results indicate that the incorporation of elastin generates aligned and more hydrophilic scaffolds with smaller fiber size. In addition, the mechanical properties of the resulting scaffolds make them adequate for use in both bioreactors and patients. All these characteristics increase the biocompatibility of these systems, generating a better interconnection with the tissue. However, due to the low maturation achieved in biological tests, no differences could be found between the incubation with and without dynamic mechanical stimulation.
骨骼肌再生变得越来越必要，这体现在越来越多的研究聚焦于改善用于此类再生的支架以及培养方案。这项工作的主要目标是通过加入弹性蛋白来改善聚己内酯（PCL）支架的特性，以实现更好的细胞增殖和生物相容性。此外，评估了两种细胞培养方案（有和没有动态机械刺激），以提高再生细胞的活性和功能产量。结果表明，弹性蛋白的加入产生了排列整齐、更具亲水性且纤维尺寸更小的支架。此外，所得支架的机械性能使其适用于生物反应器和患者。所有这些特性都增加了这些系统的生物相容性，与组织产生了更好的相互连接。然而，由于生物测试中实现的成熟度较低，在有和没有动态机械刺激的培养之间未发现差异。
