HExpPredict: Exposure Prediction of Human Blood Exposome Using a Random Forest Model and Its Application in Chemical Risk Prioritization.

BACKGROUND

Due to many substances in the human exposome, there is a dearth of exposure and toxicity information available to assess potential health risks. Quantification of all trace organics in the biological fluids seems impossible and costly, regardless of the high individual exposure variability. We hypothesized that the blood concentration () of organic pollutants could be predicted via their exposure and chemical properties. Developing a prediction model on the annotation of chemicals in human blood can provide new insight into the distribution and extent of exposures to a wide range of chemicals in humans.

OBJECTIVES

Our objective was to develop a machine learning (ML) model to predict blood concentrations () of chemicals and prioritize chemicals of health concern.

METHODS

We curated the of compounds mostly measured at population levels and developed an ML model for chemical predictions by considering chemical daily exposure (DE) and exposure pathway indicators (), half-lives (), and volume of distribution (). Three ML models, including random forest (RF), artificial neural network (ANN) and support vector regression (SVR) were compared. The toxicity potential or prioritization of each chemical was represented as a bioanalytical equivalency (BEQ) and its percentage (BEQ%) estimated based on the predicted and ToxCast bioactivity data. We also retrieved the top 25 most active chemicals in each assay to further observe changes in the BEQ% after the exclusion of the drugs and endogenous substances.

RESULTS

We curated the of 216 compounds primarily measured at population levels. RF outperformed the ANN and SVF models with the root mean square error (RMSE) of 1.66 and , the mean absolute error (MAE) values of 1.28 and , the mean absolute percentage error (MAPE) of 0.29 and 0.23, and of 0.80 and 0.72 across test and testing sets. Subsequently, the human of 7,858 ToxCast chemicals were successfully predicted, ranging from to . The predicted were then combined with ToxCast bioassays to prioritize the ToxCast chemicals across 12 assays with important toxicological end points. It is interesting that we found the most active compounds to be food additives and pesticides rather than widely monitored environmental pollutants.

DISCUSSION

We have shown that the accurate prediction of "internal exposure" from "external exposure" is possible, and this result can be quite useful in the risk prioritization. https://doi.org/10.1289/EHP11305.