Scott R C, Dugard P H, Ramsey J D, Rhodes C
Central Toxicology Laboratory, Imperial Chemical Industries PLC, Cheshire, UK.
Environ Health Perspect. 1987 Oct;74:223-7. doi: 10.1289/ehp.8774223.
The absorption of undiluted phthalate diesters [dimethyl phthalate (DMP), diethylphthalate (DEP), dibutyl phthalate (DBP) and di-(2-ethylhexyl)phthalate (DEHP)] has been measured in vitro through human and rat epidermal membranes. Epidermal membranes were set up in glass diffusion cells and their permeability to tritiated water measured to establish the integrity of the skin before the phthalate esters were applied to the epidermal surface. Absorption rates for each phthalate ester were determined and a second tritiated water permeability assessment made to quantify any irreversible alterations in barrier function due to contact with the esters. Rat skin was consistently more permeable to phthalate esters than the human skin. As the esters became more lipophilic and less hydrophilic, the rate of absorption was reduced. Contact with the esters caused little change in the barrier properties of human skin, but caused marked increases in the permeability to water of rat skin. Although differences were noted between species, the absolute rates of absorption measured indicate that the phthalate esters are slowly absorbed through both human and rat skin.
已通过人和大鼠的表皮膜在体外测量了未稀释邻苯二甲酸酯二酯[邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二乙酯（DEP）、邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）]的吸收情况。将表皮膜置于玻璃扩散池中，并测量其对氚化水的渗透性，以在将邻苯二甲酸酯应用于表皮表面之前确定皮肤的完整性。测定了每种邻苯二甲酸酯的吸收率，并进行了第二次氚化水渗透性评估，以量化由于与酯接触而导致的屏障功能的任何不可逆变化。大鼠皮肤对邻苯二甲酸酯的渗透性始终高于人类皮肤。随着酯类变得更亲脂且更不亲水，吸收速率降低。与酯接触对人类皮肤的屏障特性几乎没有影响，但导致大鼠皮肤对水的渗透性显著增加。尽管在不同物种之间观察到了差异，但所测量的绝对吸收速率表明邻苯二甲酸酯通过人和大鼠皮肤的吸收都很缓慢。
