FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA.
Molecular Education, Technology and Research Innovation Center, North Carolina State University, Raleigh, North Carolina, USA.
J Mass Spectrom. 2023 Mar;58(3):e4911. doi: 10.1002/jms.4911.
The field of mass spectrometry imaging (MSI) is constantly evolving to analyze a diverse array of biological systems. A common goal is the need to resolve cellular and subcellular heterogeneity with high spatial resolution. As the field continues to progress towards high spatial resolution, other parameters must be considered when developing a practical method. Here, we discuss the impacts of high spatial resolution on the time of acquisition and the associated implications they have on an MSI analysis (e.g., area of the region of interest). This work presents a brief tutorial serving to evaluate high spatial resolution MSI relative to time of acquisition and data file size.
质谱成像（MSI）领域不断发展，以分析各种生物系统。一个共同的目标是需要以高空间分辨率解析细胞和亚细胞异质性。随着该领域朝着高空间分辨率的方向不断发展，在开发实用方法时还必须考虑其他参数。在这里，我们讨论了高空间分辨率对采集时间的影响，以及它们对 MSI 分析（例如，感兴趣区域的面积）的影响。这项工作提供了一个简短的教程，用于评估相对于采集时间和数据文件大小的高空间分辨率 MSI。
