利用拟南芥裂琼脂系统分析根向性的实验方案。
Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana.
作者信息
Yu Bo, Zheng Wenna, Persson Staffan, Zhao Yang
机构信息
Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Department of Plant & Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
出版信息
STAR Protoc. 2023 Mar 13;4(2):102157. doi: 10.1016/j.xpro.2023.102157.
Plant roots sense salt gradients in soil to avoid saline environments through halotropism. Here, we present a protocol to study halotropism with an optimized split-agar system that simulates the salt gradient in soil. We describe steps for preparation of the split-agar system, measurement of Na, and observation of root bending. We then detail segmentation of root cells and visualization of microtubules and cellulose synthases. This system is simple to operate and has broader applications, such as hydrotropism and chemotropism. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022)..
植物根系通过向盐性感知土壤中的盐梯度，以避免处于盐碱环境。在此，我们展示了一种使用优化的分裂琼脂系统来研究向盐性的方案，该系统可模拟土壤中的盐梯度。我们描述了分裂琼脂系统的制备步骤、钠的测量以及根弯曲的观察。然后，我们详细介绍了根细胞的分割以及微管和纤维素合酶的可视化。该系统操作简单，具有更广泛的应用，如向水性和向化性。有关此方案的使用和执行的完整详细信息，请参考Yu等人（2022年）的研究。
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