Molecular Neurogenetics Unit, Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Department of Neurosurgery, Leiden University Medical Center, Leiden, the Netherlands.
Cell Rep Methods. 2023 Feb 21;3(2):100412. doi: 10.1016/j.crmeth.2023.100412. eCollection 2023 Feb 27.
Tools to effectively demonstrate and quantify functional delivery in cellular communication have been lacking. This study reports the use of a fluorescently labeled split Nanoluc reporter system to demonstrate and quantify functional transfer between cells and in a subcutaneous tumor mouse model. Our construct allows monitoring of direct, indirect, and specifically extracellular vesicle-mediated functional communication.
用于有效展示和量化细胞通讯中功能传递的工具一直缺乏。本研究报告了使用荧光标记的分裂 NanoLuc 报告系统来展示和量化细胞间以及皮下肿瘤小鼠模型中的功能转移。我们的构建体允许直接、间接和特定的细胞外囊泡介导的功能通讯的监测。
