rs671、rs429358、rs7412基因多态性不同组合与中老年高血压的关联：一项病例对照研究
Association of Different Combinations of rs671, rs429358, rs7412 Polymorphisms with Hypertension in Middle-Aged and Elderly People: A Case-Control Study.
作者信息
Lan Xinping, Wang Zhenchang, Zeng Zifeng, Yao Huaqing, Xu Weiyong, Zhang Yuxian
机构信息
Center for Cardiovascular Diseases, Meizhou People's Hospital, Meizhou Academy of Medical Sciences, Meizhou, People's Republic of China.
Guangdong Provincial Engineering and Technology Research Center for Molecular Diagnostics of Cardiovascular Diseases, Meizhou, People's Republic of China.
出版信息
Int J Gen Med. 2023 Mar 11;16:915-927. doi: 10.2147/IJGM.S402437. eCollection 2023.
BACKGROUND
Hypertensive patients have a younger trend, and studies on the role of genetic factors in hypertension susceptibility have been inconsistent. Aldehyde dehydrogenases 2 (ALDH2) and apolipoprotein E (APOE) are involved in the pathophysiological processes of hypertension. To investigate the relationship of and polymorphisms with hypertension in middle-aged (30-59 years old) and elderly (≥60 years old) persons.
METHODS
Two thousand six hundred and ten hypertensive patients and 1921 controls were included (between 30 and 100 years old). The genotypes of common polymorphisms in and genes ( rs429358, rs7412, and rs671) of the subjects were analyzed by polymerase-chain reaction (PCR)-microarray. Statistical analyses (Student's -test, Mann-Whitney -test, test, and logistic regression analysis) were performed with SPSS v21.0.
RESULTS
There were 4531 participants (66.60 ± 12.10 years old) in this study, including 3057 (67.5%) males and 1474 (32.5%) females. There were no significant differences in distributions of 2 rs671, rs429358/rs7412 genotypes and alleles between hypertensive patients and controls. Persons with rs671 G/A or A/A genotype were less likely to have hypertension (G/A+A/A vs G/G: gender-, age-, smoking-, and drinking-adjusted OR 0.885, 95% CI 0.785-0.997, =0.045), while rs671 A/A+ rs429358 or rs7412 wild-type genotype may decrease the risk of hypertension. In middle-aged group, rs671 G/A+ rs429358 T/C carriers (adjusted OR 0.547, 95% CI 0.350-0.856, =0.008), and rs671 A/A+ rs7412 C/C genotypes (adjusted OR 0.567, 95% CI 0.361-0.891, =0.014) were less likely to have hypertension. In elderly group, rs7412 T/T carriers were more likely to have hypertension (rs671 T/T vs C/C: adjusted OR 4.755, 95% CI 1.075-21.027, =0.040; rs671 T/T vs C/C or C/T: adjusted OR 4.734, 95% CI 1.071-20.928, =0.040).
CONCLUSION
Polymorphism-polymorphism interactions of rs671 and rs429358/rs7412 may effect on hypertension susceptibility. Different genotypes comparison shows different roles in middle-aged and elderly people, respectively.
背景
高血压患者有年轻化趋势，关于遗传因素在高血压易感性中作用的研究结果并不一致。乙醛脱氢酶2（ALDH2）和载脂蛋白E（APOE）参与高血压的病理生理过程。旨在探讨中年（30 - 59岁）和老年（≥60岁）人群中ALDH2和APOE基因多态性与高血压的关系。
方法
纳入2610例高血压患者和1921例对照（年龄在30至100岁之间）。采用聚合酶链反应（PCR）-微阵列法分析受试者ALDH2和APOE基因常见多态性（ALDH2 rs429358、rs7412以及APOE rs671）的基因型。使用SPSS v21.0进行统计分析（Student's t检验、Mann-Whitney U检验、χ²检验和逻辑回归分析）。
结果
本研究共有4531名参与者（66.60±12.10岁），其中男性3057名（67.5%），女性1474名（32.5%）。高血压患者与对照组之间ALDH2 rs671、APOE rs429358/rs7412基因型和等位基因分布无显著差异。APOE rs671 G/A或A/A基因型个体患高血压的可能性较小（G/A + A/A与G/G相比：经性别、年龄、吸烟和饮酒调整后的OR为0.885，95%CI为0.785 - 0.997，P = 0.045），而APOE rs671 A/A + ALDH2 rs429358或rs7412野生型基因型可能降低高血压风险。在中年组中，ALDH2 rs671 G/A + APOE rs429358 T/C携带者（调整后的OR为0.547，95%CI为0.350 - 0.856，P = 0.008）以及ALDH2 rs671 A/A + APOE rs7412 C/C基因型（调整后的OR为0.567，95%CI为0.361 - 0.891，P = 0.014）患高血压的可能性较小。在老年组中，APOE rs7412 T/T携带者患高血压的可能性较大（APOE rs671 T/T与C/C相比：调整后的OR为4.755，95%CI为1.075 - 21.027，P = 0.040；APOE rs671 T/T与C/C或C/T相比：调整后的OR为4.734，95%CI为1.071 - 20.928，P = 0.040）。
结论
ALDH2 rs671与APOE rs429358/rs7412的基因多态性相互作用可能影响高血压易感性。不同基因型比较在中年人和老年人中分别显示出不同作用。
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