Department of Otolaryngology-Head and Neck Surgery, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
Department of Surgery, Section of Otolaryngology, University of Chicago Medicine, Chicago, Illinois, USA.
Otolaryngol Head Neck Surg. 2023 Jun;168(6):1443-1452. doi: 10.1002/ohn.211. Epub 2023 Jan 24.
To examine the oral microbiome in the context of oral cavity squamous cell carcinoma.
Basic science research.
Academic medical center.
Oral swabs were collected from patients presenting to the operating room for management of oral cavity squamous cell carcinoma and from age- and sex-matched control patients receiving surgery for unrelated benign conditions. 16S ribosomal RNA (rRNA) sequencing was performed on genetic material obtained from swabs. A bacterial rRNA gene library was created and sequence reads were sorted into taxonomic units.
Thirty-one control patients (17 males) and 35 cancer patients (21 males) were enrolled. Ages ranged from 23 to 89 (median 63) for control patients and 35 to 86 (median 66) for cancer patients. Sixty-one percent of control patients and 63% of cancer patients were smokers. 16S analyses demonstrated a significant decrease in Streptococcus genera in oral cancer patients (34.11% vs 21.74% of the population, p = .04). Increases in Fusobacterium, Peptostreptococcus, Parvimonas, and Neisseria were also found. The abundance of these bacteria correlated with tumor T-stage.
16S rRNA sequencing demonstrated changes in bacterial populations in oral cavity cancer and its progression compared to noncancer controls. We found increases in bacteria genera that correspond with tumor stage-Fusobacteria, Peptostreptococcus, Parvimonas, Neisseria, and Treponema. These data suggest that oral cancer creates an environment to facilitate foreign bacterial growth, rather than implicating a specific bacterial species in carcinogenesis. These bacteria can be employed as a potential marker for tumor progression or interrogated to better characterize the tumor microenvironment.
研究口腔鳞状细胞癌患者口腔微生物组。
基础科学研究。
学术医疗中心。
从因口腔鳞状细胞癌而在手术室接受治疗的患者和因非相关良性疾病而接受手术的年龄、性别匹配的对照患者的口腔中采集口腔拭子。从拭子中提取遗传物质进行 16S 核糖体 RNA(rRNA)测序。创建细菌 rRNA 基因文库，并将序列读取结果分类到分类单元中。
共纳入 31 例对照患者（17 名男性）和 35 例癌症患者（21 名男性）。对照患者的年龄范围为 23 岁至 89 岁（中位数为 63 岁），癌症患者的年龄范围为 35 岁至 86 岁（中位数为 66 岁）。61%的对照患者和 63%的癌症患者为吸烟者。16S 分析表明，口腔癌患者口腔中链球菌属的数量显著减少（占人群的 34.11%比 21.74%，p = 0.04）。还发现梭杆菌属、消化链球菌属、小韦荣球菌属和奈瑟菌属的增加。这些细菌的丰度与肿瘤 T 分期相关。
16S rRNA 测序显示，与非癌症对照相比，口腔癌及其进展患者的细菌种群发生了变化。我们发现与肿瘤分期相关的细菌属增加，包括梭杆菌属、消化链球菌属、小韦荣球菌属、奈瑟菌属和密螺旋体属。这些数据表明，口腔癌创造了有利于外来细菌生长的环境，而不是暗示特定的细菌物种与癌变有关。这些细菌可以作为肿瘤进展的潜在标志物，或用于更好地描述肿瘤微环境。
