Zhu Yanlin, Zhao Ruoxi, Feng Lili, Wang Chen, Dong Shuming, Zyuzin Mikhail V, Timin Alexander, Hu Narisu, Liu Bin, Yang Piaoping
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, P. R. China.
Department of Research, Guangxi Medical University Cancer Hospital, Nanning 530021, P. R. China.
ACS Nano. 2023 Apr 11;17(7):6833-6848. doi: 10.1021/acsnano.3c00423. Epub 2023 Mar 28.
Specific generation of reactive oxygen species (ROS) within tumors catalyzed by nanozymes is a promising strategy for cancer therapeutics. However, it remains a significant challenge to fabricate highly efficient nanozymes acting in the tumor microenvironment. Herein, we develop a bimetallic nanozyme (PtSn) with the photothermal enhancement of dual enzymatic activities for tumor catalytic therapy. The structures and activities of PtSn bimetallic nanoclusters (BNCs) with different Sn content are explored and evaluated systematically. Experimental comparisons show that the PtSn BNCs exhibit the highest activities among all those investigated, including enzymatic activity and photothermal property, due to the generation of SnO with oxygen vacancy (O) sites on the surface of PtSn BNCs. Specifically, the PtSn BNCs exhibit photothermal-enhanced peroxidase-like and catalase-like activities, as well as a significantly enhanced anticancer efficacy in both multicellular tumor spheroids and experiments. Due to the high X-ray attenuation coefficient and excellent light absorption property, the PtSn BNCs also show dual-mode imaging capacity of computed tomography and photoacoustic imaging, which could achieve real-time monitoring of the therapeutic process. Therefore, this work will advance the development of noble-metal nanozymes with optimal composition for efficient tumor catalytic therapy.
由纳米酶催化在肿瘤内特异性产生活性氧（ROS）是一种很有前景的癌症治疗策略。然而，制备在肿瘤微环境中起作用的高效纳米酶仍然是一项重大挑战。在此，我们开发了一种具有光热增强双重酶活性的双金属纳米酶（PtSn）用于肿瘤催化治疗。系统地探索和评估了不同Sn含量的PtSn双金属纳米簇（BNCs）的结构和活性。实验比较表明，由于PtSn BNCs表面产生了具有氧空位（O）位点的SnO，PtSn BNCs在所有研究对象中表现出最高的活性，包括酶活性和光热性能。具体而言，PtSn BNCs表现出光热增强的过氧化物酶样和过氧化氢酶样活性，以及在多细胞肿瘤球体和实验中显著增强的抗癌效果。由于高X射线衰减系数和优异的光吸收性能，PtSn BNCs还显示出计算机断层扫描和光声成像的双模成像能力，可实现对治疗过程的实时监测。因此，这项工作将推动具有最佳组成的贵金属纳米酶用于高效肿瘤催化治疗的发展。
