Ravindran Girija Aswathy, Strudwick Xanthe, Balasubramanian Sivakumar, Palaninathan Vivekanandan, Nair Sakthikumar Dasappan, Cowin Allison J
Future Industries Institute, University of South Australia, Mawson Lakes, SA 5095, Australia.
Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe 350-0815, Saitama, Japan.
Pharmaceutics. 2023 Mar 8;15(3):880. doi: 10.3390/pharmaceutics15030880.
Electrospun fibers are widely studied in regenerative medicine for their ability to mimic the extracellular matrix (ECM) and provide mechanical support. In vitro studies indicated that cell adhesion and migration is superior on smooth poly(L-lactic acid) (PLLA) electrospun scaffolds and porous scaffolds once biofunctionalized with collagen.
The in vivo performance of PLLA scaffolds with modified topology and collagen biofunctionalization in full-thickness mouse wounds was assessed by cellular infiltration, wound closure and re-epithelialization and ECM deposition.
Early indications suggested unmodified, smooth PLLA scaffolds perform poorly, with limited cellular infiltration and matrix deposition around the scaffold, the largest wound area, a significantly larger panniculus gape, and lowest re-epithelialization; however, by day 14, no significant differences were observed. Collagen biofunctionalization may improve healing, as collagen-functionalized smooth scaffolds were smallest overall, and collagen-functionalized porous scaffolds were smaller than non-functionalized porous scaffolds; the highest re-epithelialization was observed in wounds treated with collagen-functionalized scaffolds.
Our results suggest that limited incorporation of smooth PLLA scaffolds into the healing wound occurs, and that altering surface topology, particularly by utilizing collagen biofunctionalization, may improve healing. The differing performance of the unmodified scaffolds in the in vitro versus in vivo studies demonstrates the importance of preclinical testing.
电纺纤维因其能够模拟细胞外基质（ECM）并提供机械支持而在再生医学中得到广泛研究。体外研究表明，在光滑的聚（L-乳酸）（PLLA）电纺支架以及用胶原蛋白进行生物功能化后的多孔支架上，细胞黏附和迁移能力更强。
通过细胞浸润、伤口闭合、再上皮化以及ECM沉积，评估具有改良拓扑结构和胶原蛋白生物功能化的PLLA支架在小鼠全层伤口中的体内性能。
早期迹象表明，未改性的光滑PLLA支架表现不佳，支架周围的细胞浸润和基质沉积有限，伤口面积最大， panniculus间隙明显更大，再上皮化程度最低；然而，到第14天，未观察到显著差异。胶原蛋白生物功能化可能会促进愈合，因为胶原蛋白功能化的光滑支架总体上最小，胶原蛋白功能化的多孔支架比未功能化的多孔支架更小；在用胶原蛋白功能化支架治疗的伤口中观察到最高的再上皮化程度。
我们的结果表明，光滑的PLLA支架在愈合伤口中的整合有限，改变表面拓扑结构，特别是通过利用胶原蛋白生物功能化，可能会促进愈合。未改性支架在体外和体内研究中的不同表现证明了临床前测试的重要性。
