Budiawan Antonius, Purwanto Agus, Puradewa Levi, Cahyani Erlien Dwi, Purwaningsih Christiana Endang
Pharmacy Diploma III Department, Widya Mandala Surabaya Catholic University Manggis 15-17 Madiun City 63131 East Java Indonesia
Biology Department, Widya Mandala Surabaya Catholic University Manggis 15-17 Madiun City 63131 East Java Indonesia.
RSC Adv. 2023 Mar 28;13(15):9871-9877. doi: 10.1039/d3ra00868a. eCollection 2023 Mar 27.
Purslane has various varieties with different active metabolite contents that need to be explored further to find each variety's activity in wound healing. Different purslane herbs showed different antioxidant activities, suggesting they will have different flavonoid content and wound healing activity. This research aimed to evaluate purslane's total flavonoid content and wound-healing activity. The wounds induced on the rabbit back skin were divided into 6 treatment groups such as negative control, positive control, 10 and 20% purslane herbs extract varieties A, and 10 and 20% purslane herbs extract varieties C. Wounds were treated twice daily for 2 weeks, and measured on day 0, 7, 11, and 14. Total flavonoid content was measured with the AlCl colorimetric method. The wounds treated with 10 and 20% purslane herbs extract varieties A ( magenta flower) have 0.32 ± 0.55 and 1.63 ± 1.96 mm wound diameters on day 7 and healed on day 11. The wounds treated with 10 and 20% purslane herbs extract varieties C ( pink flower) showed 2.88 ± 0.51 and 0.84 ± 1.45 mm diameter and healed on day 11. The purslane herb A showed the highest wound healing activity, and purslane varieties A and C total flavonoid contents were 0.55 ± 0.02 and 1.58 ± 0.02% w/w, respectively.
马齿苋有多种品种，其活性代谢物含量各不相同，需要进一步探索以发现每个品种在伤口愈合方面的活性。不同的马齿苋草药表现出不同的抗氧化活性，这表明它们的黄酮类化合物含量和伤口愈合活性也会有所不同。本研究旨在评估马齿苋的总黄酮含量和伤口愈合活性。在兔背部皮肤上诱导的伤口被分为6个治疗组，即阴性对照组、阳性对照组、10%和20%的马齿苋品种A草药提取物组，以及10%和20%的马齿苋品种C草药提取物组。伤口每天治疗两次，持续2周，并在第0、7、11和14天进行测量。总黄酮含量采用氯化铝比色法测定。用10%和20%马齿苋品种A（紫红色花）草药提取物治疗的伤口在第7天的伤口直径分别为0.32±0.55毫米和1.63±1.96毫米，并在第11天愈合。用10%和20%马齿苋品种C（粉红色花）草药提取物治疗的伤口直径分别为2.88±0.51毫米和0.84±1.45毫米，并在第11天愈合。马齿苋品种A表现出最高的伤口愈合活性，马齿苋品种A和C的总黄酮含量分别为0.55±0.02%（w/w）和1.58±0.02%（w/w）。
