Baseman J B, Nichols J C, Mogerley S
Infect Immun. 1979 Feb;23(2):392-7. doi: 10.1128/iai.23.2.392-397.1979.
Treponema pallidum (Nichols) was extracted from infected rabbit tissue, and cell lysates were prepared for monitoring thymidine kinase and deoxyribonucleic acid polymerase activities. No thymidine kinase could be demonstrated in preparations of T. pallidum or the cultivable T. phagedenis biotype Reiter. Significant levels of deoxyribonucleic acid polymerase were detected in both treponemal samples. Interestingly, comparisons of polymerase activity among a spectrum of bacterial genera revealed a direct correlation between enzyme concentrations and estimated generation time. Incorporation of [3H]uridine and [3H]thymidine into macromolecules by intact T. pallidum and the Reiter treponeme was examined. Selective ribonuclease-deoxyribonuclease digestion and cesium chloride gradient banding demonstrated that T. pallidum, independent of the host, and T. phagedenis were capable of synthesizing deoxyribonucleic acid only from the [3H]-uridine precursor.
从感染的兔组织中提取梅毒螺旋体（Nichols株），并制备细胞裂解物以监测胸苷激酶和脱氧核糖核酸聚合酶的活性。在梅毒螺旋体制备物或可培养的溶牙螺旋体生物型赖特氏螺旋体中均未检测到胸苷激酶。在两种密螺旋体样品中均检测到显著水平的脱氧核糖核酸聚合酶。有趣的是，对一系列细菌属之间的聚合酶活性进行比较发现，酶浓度与估计的代时之间存在直接相关性。研究了完整的梅毒螺旋体和赖特氏密螺旋体将[3H]尿苷和[3H]胸苷掺入大分子的情况。选择性核糖核酸酶-脱氧核糖核酸酶消化和氯化铯梯度带谱分析表明，梅毒螺旋体（不依赖宿主）和溶牙螺旋体仅能够从[3H]尿苷前体合成脱氧核糖核酸。
