Kusumoto Masahiro, Tamamura-Andoh Yukino, Hikoda-Kogiku Yuna, Magome Asami, Okuhama Erina, Sato Keisuke, Mizuno Yoshino, Arai Nobuo, Watanabe-Yanai Ayako, Iwata Taketoshi, Ogura Yoshitoshi, Gotoh Yasuhiro, Nakamura Keiji, Hayashi Tetsuya, Akiba Masato
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Japan.
Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka, Japan.
Front Microbiol. 2023 Mar 17;14:1107566. doi: 10.3389/fmicb.2023.1107566. eCollection 2023.
Pathogenic strains are important causes of several swine diseases that result in significant economic losses worldwide. In Japan, the use of antimicrobials in swine is much higher than that in other farm animals every year. Antimicrobial resistance in pathogenic strains also heavily impacts the swine industry due to the limited treatment options and an increase in the potential risk of the One Health crisis. In 2016, we investigated 684 Japanese isolates of swine pathogenic belonging to four major serogroups and reported the emergence and increase in the highly multidrug-resistant serogroups O116 and OSB9 and the appearance of colistin-resistant strains. In the present study, by expanding our previous analysis, we determined the serotypes and antimicrobial resistance of 1,708 strains isolated from diseased swine between 1991 and 2019 in Japan and found recent increases in the prevalences of multidrug-resistant strains and minor serogroup strains. Among the antimicrobials examined in this study that have been approved for animal use, a third-generation cephalosporin was found to be effective against the most isolates (resistance rate: 1.2%) but not against highly multidrug-resistant strains. We also analyzed the susceptibilities of the 1,708 isolates to apramycin and bicozamycin, both which are available for treating swine in Japan, and found that the rates of resistance to apramycin and bicozamycin were low (6.7% and 5.8%, respectively), and both antimicrobials are more effective (resistance rates: 2.7% and 5.4%, respectively) than third-generation cephalosporins (resistance rate: 16.2%) against highly multidrug-resistant strains.
致病性菌株是导致多种猪病的重要原因，在全球范围内造成了重大经济损失。在日本，猪用抗菌药物的使用量每年都远高于其他农场动物。由于治疗选择有限以及“同一健康”危机潜在风险的增加，致病性菌株中的抗菌药物耐药性也对养猪业产生了严重影响。2016年，我们调查了684株来自日本的猪致病性菌株，它们分属于四个主要血清群，并报告了高度多重耐药血清群O116和OSB9的出现及增加，以及耐黏菌素菌株的出现。在本研究中，通过扩展我们之前的分析，我们确定了1991年至2019年期间从日本患病猪分离出的1708株菌株的血清型和抗菌药物耐药性，发现多重耐药菌株和次要血清群菌株的流行率最近有所增加。在本研究中检测的已获批准用于动物的抗菌药物中，发现第三代头孢菌素对大多数分离株有效（耐药率：1.2%），但对高度多重耐药菌株无效。我们还分析了这1708株分离株对阿普拉霉素和比考扎霉素的敏感性，这两种药物在日本均可用于治疗猪，发现对阿普拉霉素和比考扎霉素的耐药率较低（分别为6.7%和5.8%），并且这两种抗菌药物对高度多重耐药菌株比第三代头孢菌素更有效（耐药率分别为2.7%和5.4%，而第三代头孢菌素的耐药率为16.2%）。
