Karaca Nursenem, Demirci Betül, Gavahian Mohsen, Demirci Fatih
Department of Pharmacognosy, Graduate School of Health Sciences, Anadolu University, Eskisehir 26470, Türkiye.
Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir 26470, Türkiye.
ACS Omega. 2023 Mar 14;8(12):10941-10953. doi: 10.1021/acsomega.2c07508. eCollection 2023 Mar 28.
This study aimed to increase the bioactivity of essential oils by fractionation, combination, and emulsification. In this regard, pharmaceutical quality L. (rosemary), L. (clary sage), Medik. (spike lavender), and L. (chamomile) essential oils were fractionated by vacuum-column chromatography. The main components of the essential oils were verified, and their fractions were characterized by thin layer chromatography, gas chromatography-flame ionization detector, and gas chromatography/mass spectrometry. Besides, oil-in-water (O/W) emulsions of essential oils and diethyl ether fractions were obtained by the self-emulsification method, followed by droplet size, polydispersity index, and zeta potential value measurements. The in vitro antibacterial effects of the emulsions and binary combinations (10:90, 20:80, 30:70, 40:60, 50:50, 60:40, 70:30, 80:20, 90:10, v:v) against were determined by microdilution. In addition, the in vitro anti-biofilm, antioxidant, and anti-inflammatory effects of emulsion formulations were evaluated. According to the experimental results, fractionation and emulsification enhanced essential oil in vitro antibacterial, anti-inflammatory, and antioxidant effects due to increased solubility and nano-sized droplets. Among 22 different emulsion combinations, 1584 test concentrations resulted in 21 cases of synergistic effects. The mechanism of the increase in biological activities was hypothesized to be higher solubility and stability of the essential oil fractions. Food and pharmaceutical industries may benefit from the procedure proposed in this study.
本研究旨在通过分馏、混合和乳化提高精油的生物活性。在这方面，采用真空柱色谱法对药用品质的迷迭香叶、鼠尾草叶、穗花薰衣草和母菊花精油进行分馏。对精油的主要成分进行了验证，并通过薄层色谱、气相色谱 - 火焰离子化检测器和气相色谱/质谱对其馏分进行了表征。此外，通过自乳化法获得了精油和二乙醚馏分的水包油（O/W）乳液，随后测量了液滴大小、多分散指数和ζ电位值。通过微量稀释法测定了乳液和二元组合（10:90、20:80、30:70、40:60、50:50、60:40、70:30、80:20、90:10，v:v）对[具体细菌名称未给出]的体外抗菌效果。此外，还评估了乳液制剂的体外抗生物膜、抗氧化和抗炎作用。根据实验结果，分馏和乳化由于增加了溶解度和纳米级液滴，增强了精油的体外抗菌、抗炎和抗氧化作用。在22种不同的乳液组合中，1584个测试浓度产生了21例协同效应。生物活性增加的机制被推测为精油馏分具有更高的溶解度和稳定性。食品和制药行业可能会从本研究提出的方法中受益。
