Qian Xu, Jin Hui, Chen Zhuojun, Dai Qingqing, Sarsaiya Surendra, Qin Yitong, Jia Qi, Jin Leilei, Chen Jishuang
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
Bioresource Institute for Healthy Utilization, Zunyi Medical University, Zunyi, China.
Front Microbiol. 2021 Dec 15;12:800125. doi: 10.3389/fmicb.2021.800125. eCollection 2021.
MD33, a sesquiterpene alkaloid-producing endophyte isolated from , shows potential medical and industrial applications. To understand the molecular mechanisms of sesquiterpene alkaloids production, a comparative transcriptome analysis was performed on strain MD33 and its positive mutant UN32, which was created using Ultraviolet (UV) mutagenesis and nitrogen ion (N) implantation. The alkaloid production of UN32 was 2.62 times more than that of MD33. One thousand twenty-four differentially expressed genes (DEGs), including 519 up-regulated and 505 down-regulated genes, were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 139 GO terms and 87 biosynthesis pathways. Dendrobine, arguably the main sesquiterpene alkaloid the strain MD33 produced, might start synthesis through the mevalonate (MVA) pathway. Several MVA pathway enzyme-coding genes (hydroxy-methylglutaryl-CoA synthase, mevalonate kinase, and farnesyl diphosphate synthase) were found to be differentially expressed, suggesting that physical mutagenesis can disrupt genome integrity and gene expression. Some backbone post-modification enzymes and transcript factors were either discovered, suggesting the sesquiterpene alkaloid metabolism in is a complex genetic network. Our findings help to shed light on the underlying molecular regulatory mechanism of sesquiterpene alkaloids production in .
MD33是一种从[具体来源未给出]中分离出的能产生倍半萜生物碱的内生菌，具有潜在的医学和工业应用价值。为了解倍半萜生物碱产生的分子机制，对菌株MD33及其通过紫外线（UV）诱变和氮离子（N）注入产生的正向突变体UN32进行了比较转录组分析。UN32的生物碱产量是MD33的2.62倍。共鉴定出1024个差异表达基因（DEG），其中包括519个上调基因和505个下调基因。基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析揭示了139个GO术语和87条生物合成途径。可认为是菌株MD33产生的主要倍半萜生物碱的石蒜碱，可能通过甲羟戊酸（MVA）途径开始合成。发现几个MVA途径的酶编码基因（羟甲基戊二酰辅酶A合酶、甲羟戊酸激酶和法尼基二磷酸合酶）存在差异表达，表明物理诱变可破坏基因组完整性和基因表达。还发现了一些骨架后修饰酶和转录因子，表明[具体物种未给出]中的倍半萜生物碱代谢是一个复杂的遗传网络。我们的研究结果有助于揭示[具体物种未给出]中倍半萜生物碱产生的潜在分子调控机制。
