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基于分子印迹聚吡咯的用于细菌检测的电化学传感器研究进展

Towards Electrochemical Sensor Based on Molecularly Imprinted Polypyrrole for the Detection of Bacteria-.

作者信息

Liustrovaite Viktorija, Pogorielov Maksym, Boguzaite Raimonda, Ratautaite Vilma, Ramanaviciene Almira, Pilvenyte Greta, Holubnycha Viktoriia, Korniienko Viktoriia, Diedkova Kateryna, Viter Roman, Ramanavicius Arunas

机构信息

Department of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.

Biomedical Research Centre, Sumy State University, R-Korsakova Street, 40007 Sumy, Ukraine.

出版信息

Polymers (Basel). 2023 Mar 23;15(7):1597. doi: 10.3390/polym15071597.

DOI:10.3390/polym15071597
PMID:37050211
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10097406/
Abstract

Detecting bacteria--is an essential healthcare and food industry issue. The objective of the current study was to apply platinum (Pt) and screen-printed carbon (SPCE) electrodes modified by molecularly imprinted polymer (MIP) in the design of an electrochemical sensor for the detection of . A sequence of potential pulses was used to perform the electrochemical deposition of the non-imprinted polypyrrole (NIP-Ppy) layer and -imprinted polypyrrole (MIP-Ppy) layer over SPCE and Pt electrodes. The bacteria were removed by incubating Ppy-modified electrodes in different extraction solutions (sulphuric acid, acetic acid, L-lysine, and trypsin) to determine the most efficient solution for extraction and to obtain a more sensitive and repeatable design of the sensor. The performance of MIP-Ppy- and NIP-Ppy-modified electrodes was evaluated by pulsed amperometric detection (PAD). According to the results of this research, it can be assumed that the most effective MIP-Ppy/SPCE sensor can be designed by removing bacteria with the proteolytic enzyme trypsin. The LOD and LOQ of the MIP-Ppy/SPCE were 70 CFU/mL and 210 CFU/mL, respectively, with a linear range from 300 to 6700 CFU/mL.

摘要

检测细菌是医疗保健和食品行业的一个重要问题。本研究的目的是将铂（Pt）和分子印迹聚合物（MIP）修饰的丝网印刷碳（SPCE）电极应用于电化学传感器的设计中，用于检测[此处原文缺失检测对象]。使用一系列电位脉冲在SPCE和Pt电极上进行非印迹聚吡咯（NIP-Ppy）层和[此处原文缺失内容]印迹聚吡咯（MIP-Ppy）层的电化学沉积。通过将聚吡咯修饰的电极在不同的萃取溶液（硫酸、乙酸、L-赖氨酸和胰蛋白酶）中孵育来去除细菌，以确定最有效的萃取溶液，并获得更灵敏且可重复的传感器设计。通过脉冲安培检测（PAD）评估MIP-Ppy和NIP-Ppy修饰电极的性能。根据本研究结果，可以推测通过用蛋白水解酶胰蛋白酶去除细菌能够设计出最有效的MIP-Ppy/SPCE传感器。MIP-Ppy/SPCE的检测限（LOD）和定量限（LOQ）分别为70 CFU/mL和210 CFU/mL，线性范围为300至6700 CFU/mL。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/04aca97c5c59/polymers-15-01597-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/9e357e1b08b3/polymers-15-01597-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/88d03e064a9c/polymers-15-01597-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/df4efdc62bd0/polymers-15-01597-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/cd5bab4755b7/polymers-15-01597-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/769ed294e615/polymers-15-01597-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/1b93a630fa52/polymers-15-01597-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/a57d3eb924d7/polymers-15-01597-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/04aca97c5c59/polymers-15-01597-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/9e357e1b08b3/polymers-15-01597-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/88d03e064a9c/polymers-15-01597-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/df4efdc62bd0/polymers-15-01597-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/cd5bab4755b7/polymers-15-01597-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/769ed294e615/polymers-15-01597-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/1b93a630fa52/polymers-15-01597-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/a57d3eb924d7/polymers-15-01597-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b00/10097406/04aca97c5c59/polymers-15-01597-g007.jpg

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2
An study on the degradation of multispecies biofilm of periodontitis-related microorganisms by bovine trypsin.一项关于牛胰蛋白酶对牙周炎相关微生物多物种生物膜降解作用的研究。
Front Microbiol. 2022 Aug 4;13:951291. doi: 10.3389/fmicb.2022.951291. eCollection 2022.
3
Molecularly imprinted polymers in diagnostics: accessing analytes in biofluids.
Compr Rev Food Sci Food Saf. 2025 Mar;24(2):e70124. doi: 10.1111/1541-4337.70124.
4
Molecular Imprinting Technology for Advanced Delivery of Essential Oils.用于精油高级递送的分子印迹技术
Polymers (Basel). 2024 Aug 28;16(17):2441. doi: 10.3390/polym16172441.
5
Emerging Biomimetic Sensor Technologies for the Detection of Pathogenic Bacteria: A Commercial Viability Study.用于检测病原菌的新兴仿生传感器技术：商业可行性研究。
ACS Omega. 2024 May 23;9(22):23155-23171. doi: 10.1021/acsomega.4c01478. eCollection 2024 Jun 4.
6
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Biosensors (Basel). 2023 Jun 5;13(6):620. doi: 10.3390/bios13060620.
诊断中的分子印迹聚合物：获取生物流体中的分析物。
J Mater Chem B. 2022 Sep 28;10(37):7418-7449. doi: 10.1039/d2tb00703g.
4
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5
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infections: presentation, diagnosis and treatment.感染：表现、诊断与治疗。
Br J Hosp Med (Lond). 2021 Oct 2;82(10):1-6. doi: 10.12968/hmed.2021.0107. Epub 2021 Oct 5.
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