Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Carbohydr Polym. 2023 Jul 15;312:120787. doi: 10.1016/j.carbpol.2023.120787. Epub 2023 Mar 9.
Three-dimensional (3D) printing technology has become an advanced approach for fabricating patient-specific scaffolds with complex geometric shapes to replace damaged or diseased tissue. Herein, polylactic acid (PLA)-Baghdadite (Bgh) scaffold were made through the fused deposition modeling (FDM) 3D printing method and subjected to alkaline treatment. Following fabrication, the scaffolds were coated with either chitosan (Cs)-vascular endothelial growth factor (VEGF) or lyophilized Cs-VEGF known as PLA-Bgh/Cs-VEGF and PLA-Bgh/L.(Cs-VEGF), respectively. Based on the results, it was found that the coated scaffolds had higher porosity, compressive strength and elastic modulus than PLA and PLA-Bgh samples. Also, the osteogenic differentiation potential of scaffolds following culture with rat bone marrow-derived mesenchymal stem cells (rMSCs) was evaluated through crystal violet and Alizarin-red staining, alkaline phosphatase (ALP) activity and calcium content assays, osteocalcin measurements, and gene expression analysis. The release of VEGF from the coated scaffolds was assessed and also the angiogenic potential of scaffolds was evaluated. The sum of results presented in the current study strongly suggests that the PLA-Bgh/L.(Cs-VEGF) scaffold can be a proper candidate for bone healing applications.
三维（3D）打印技术已成为制造具有复杂几何形状的患者特异性支架的先进方法，以替代受损或患病的组织。本文通过熔融沉积建模（FDM）3D 打印方法制造了聚乳酸（PLA）-Baghdadite（Bgh）支架，并进行了碱性处理。制造后，支架分别用壳聚糖（Cs）-血管内皮生长因子（VEGF）或冻干的 Cs-VEGF 进行涂层，分别称为 PLA-Bgh/Cs-VEGF 和 PLA-Bgh/L.(Cs-VEGF)。结果表明，涂层支架的孔隙率、压缩强度和弹性模量均高于 PLA 和 PLA-Bgh 样品。此外，通过结晶紫和茜素红染色、碱性磷酸酶（ALP）活性和钙含量测定、骨钙素测量以及基因表达分析，评估了支架在与大鼠骨髓间充质干细胞（rMSCs）共培养后的成骨分化潜力。评估了涂层支架中 VEGF 的释放以及支架的血管生成潜力。本研究的结果表明，PLA-Bgh/L.(Cs-VEGF) 支架可能是骨愈合应用的合适候选者。
