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基因组报告:非洲刺毛鼠的染色体水平基因组组装( )。

GENOME REPORT: Chromosome-scale genome assembly of the African spiny mouse ( ).

作者信息

Nguyen Elizabeth Dong, Fard Vahid Nikoonejad, Kim Bernard Y, Collins Sarah, Galey Miranda, Nelson Branden R, Wakenight Paul, Gable Simone M, McKenna Aaron, Bammler Theo K, MacDonald Jim, Okamura Daryl M, Shendure Jay, Beier David R, Ramirez Jan Marino, Majesky Mark W, Millen Kathleen J, Tollis Marc, Miller Danny E

机构信息

Department of Pediatrics, University of Washington, Seattle, WA.

Center for Developmental Biology & Regenerative Medicine, Seattle Children's Research Institute, Seattle, WA.

出版信息

bioRxiv. 2023 Apr 5:2023.04.03.535372. doi: 10.1101/2023.04.03.535372.

Abstract

There is increasing interest in the African spiny mouse ( ) as a model organism because of its ability for regeneration of tissue after injury in skin, muscle, and internal organs such as the kidneys. A high-quality reference genome is needed to better understand these regenerative properties at the molecular level. Here, we present an improved reference genome for generated from long Nanopore sequencing reads. We confirm the quality of our annotations using RNA sequencing data from four different tissues. Our genome is of higher contiguity and quality than previously reported genomes from this species and will facilitate ongoing efforts to better understand the regenerative properties of this organism.

摘要

由于非洲刺毛鼠在皮肤、肌肉以及肾脏等内部器官受伤后具有组织再生能力,因此它作为一种模式生物越来越受到关注。为了在分子水平上更好地理解这些再生特性,需要一个高质量的参考基因组。在此,我们展示了一个基于长纳米孔测序读数生成的改进的非洲刺毛鼠参考基因组。我们使用来自四种不同组织的RNA测序数据确认了注释的质量。我们的基因组比该物种先前报道的基因组具有更高的连续性和质量,将有助于正在进行的更好地理解这种生物再生特性的研究工作。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65d3/10103962/b2d124ab5f0c/nihpp-2023.04.03.535372v1-f0001.jpg

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