Yimer Abebe, Forsido Sirawdink Fikereyesus, Addis Getachew, Ayelign Abebe
Department of Post-Harvest Management, College of Agriculture and Veterinary Medicine, Jimma University, P.O. Box: 307, Jimma, Ethiopia.
Ethiopian Public Health Institute, P.O. Box: 1242 Addis Ababa, Ethiopia.
Heliyon. 2023 Apr 7;9(4):e15331. doi: 10.1016/j.heliyon.2023.e15331. eCollection 2023 Apr.
Wild edible plants (WEPs) such as L., A. Rich., Benth., DC., and L. are widely used for various forms of culinary and folk medicine in Southwest Ethiopia. However, the phytochemical content of these plants is not explored. Thus, this study aimed to determine the total phenols, flavonoids, antioxidants, vitamin C, and β-carotene in edible parts of the plants. Edible parts were oven-dried and extracted with methanol. Total phenolic content (TPC) and total flavonoid content (TFC) were determined using Folin Ciocalteu and Aluminium chloride colorimetric methods, respectively. antioxidant activities were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) tests. β-carotene and vitamin C content were assessed using spectrophotometric and titration, respectively. TPC ranged from 0.25 ± 0.06 mg GAE/g in tuber to 35.73 ± 2.52 mg GAE/g in leaf, while TFC varied from 0.85 ± 0.03 to 11.25 ± 0.01 mg CE/g in tuber and leaf. In the DPPH assay, the antioxidant value ranged from 50.09% in tuber to 87.63% in leaf; while in the FRAP assay, the value ranged from 49.16 ± 2.13 in tuber to 188.12 ± 1.13 mM Fe/100 g in leaf. Similarly, β-carotene content was recorded between 11.81 ± 0.00 mg/100g in tuber to 34.49 ± 0.95 mg/100g in leaf. The concentration of vitamin C ranged from 10.00 ± 0.61 in tuber to 45 ± 1.80 mg/100g in leaf. The results showed strong positive correlations between FRAP and TPC (r = 0.94), and FRAP and vitamin C (r = 0.93). and contain abundant levels of TPC and TFC. leaf contains a good source of vitamin C and β-carotene. These WEPs contribute a natural supply of dietary antioxidants that prevent oxidative stress.
诸如[具体植物名称1]、[具体植物名称2]、[具体植物名称3]、[具体植物名称4]和[具体植物名称5]等野生可食用植物在埃塞俄比亚西南部被广泛用于各种烹饪和民间医药用途。然而，这些植物的植物化学成分尚未得到研究。因此，本研究旨在测定这些植物可食用部分中的总酚、黄酮类化合物、抗氧化剂、维生素C和β-胡萝卜素。可食用部分经烘干后用甲醇提取。分别采用福林-酚法和氯化铝比色法测定总酚含量（TPC）和总黄酮含量（TFC）。采用2,2-二苯基-1-苦基肼（DPPH）和铁还原抗氧化能力（FRAP）试验评估抗氧化活性。分别采用分光光度法和滴定法评估β-胡萝卜素和维生素C含量。TPC范围从[具体植物名称1]块茎中的0.25±0.06毫克没食子酸当量/克到[具体植物名称1]叶片中的35.73±2.52毫克没食子酸当量/克，而TFC在[具体植物名称1]块茎和叶片中分别为0.85±0.03至11.25±0.01毫克儿茶素当量/克。在DPPH试验中，抗氧化值范围从[具体植物名称1]块茎中的50.09%到[具体植物名称1]叶片中的87.63%；而在FRAP试验中，该值范围从[具体植物名称1]块茎中的49.16±2.13到[具体植物名称1]叶片中的188.12±1.13毫摩尔铁/100克。同样，β-胡萝卜素含量在[具体植物名称1]块茎中为11.81±0.00毫克/100克至[具体植物名称1]叶片中的34.49±0.95毫克/100克。维生素C浓度范围从[具体植物名称1]块茎中的10.00±0.61到[具体植物名称1]叶片中的45±1.80毫克/100克。结果显示FRAP与TPC之间存在强正相关（r = 0.94），以及FRAP与维生素C之间存在强正相关（r = 0.93）。[具体植物名称1]和[具体植物名称2]含有丰富的TPC和TFC。[具体植物名称1]叶片含有丰富的维生素C和β-胡萝卜素来源。这些野生可食用植物为预防氧化应激提供了天然的膳食抗氧化剂供应。
