Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Araraquara, 14800900, Brazil.
Department of Dental Materials and Prosthodontics, School of Dentistry, University of São Paulo (USP), Ribeirão Preto, 14049-900, Brazil.
J Mater Sci Mater Med. 2023 Apr 28;34(5):21. doi: 10.1007/s10856-023-06717-3.
High implant survival rates have been achieved in recent decades due to continual modifications in implant design and surface topography, however there is still an ongoing quest to control peri-implant bone loss. The objective of this work was to develop Ti-35Nb-7Zr-5Ta (TNZT) alloys, perform physicochemical and morphological characterization of their surface modified by electrolytic oxidative plasma technique with ions related to osseointegration and lastly evaluate bacterial colonization in vitro. Three groups were evaluated: C group (polished TNZT), CaP group (sodium β glycerophosphate + calcium acetate) and Mg group (magnesium acetate). Before and after anodizing the surfaces, physicochemical and morphological analyses were performed: scanning electron microscopy with field emission gun (FEG-SEM), energy dispersion spectroscopy (EDS), X-ray diffraction (DRX), wettability (goniometer) and roughness (rugometer). Controlled and treated specimens were contaminated with unstimulated saliva collected from 10 healthy volunteers. Then, biofilm samples were collected and up to 35 microbial species, including commensal and pathogenic microorganisms, were identified and quantified by the Checkerboard DNA-DNA Hybridization method. The CaP group modified the surface morphology in the form of pores, while the Mg group modified it in the form of flakes. The contact angle was significantly smaller in the CaP group. The average roughness was higher in the CaP and Mg groups. A smaller total amount of bacteria was identified in the Mg group and relevant differences were found in the microbial profile associated with different surface treatments. Therefore, considering the microbiological profile and for the prevention of peri-implantitis, the Mg group presented more satisfactory and encouraging results for the manufacture of dental implants.
近几十年来，由于不断改进种植体设计和表面形貌，种植体的存活率已经很高，但仍在不断探索控制种植体周围骨丢失的方法。本工作旨在开发 Ti-35Nb-7Zr-5Ta（TNZT）合金，通过与骨整合相关的离子对其进行电解氧化等离子体技术表面改性，并对其进行理化和形态特征分析，最后进行体外细菌定植评估。评估了三组：C 组（抛光 TNZT）、CaP 组（β-甘油磷酸钠+醋酸钙）和 Mg 组（醋酸镁）。在阳极氧化前后，对表面进行理化和形态分析：场发射枪扫描电子显微镜（FEG-SEM）、能谱（EDS）、X 射线衍射（DRX）、润湿性（接触角仪）和粗糙度（粗糙度仪）。对照和处理后的标本用从 10 名健康志愿者中收集的未刺激唾液污染。然后，用棋盘式 DNA-DNA 杂交法收集生物膜样本，鉴定和定量多达 35 种微生物，包括共生和致病微生物。CaP 组以孔的形式改变表面形貌，而 Mg 组以薄片的形式改变表面形貌。CaP 组的接触角显著减小。CaP 和 Mg 组的平均粗糙度较高。Mg 组中鉴定出的总细菌量较少，不同表面处理相关的微生物谱存在差异。因此，考虑到微生物谱，为了预防种植体周围炎，Mg 组在制造牙科种植体方面表现出更令人满意和有希望的结果。
