Department of Cardiovascular Medicine, Central Hospital Affiliated to Shandong First Medical University, Jinan Central Hospital, Jinan, Shandong 250000, P.R. China.
Dalian Medical University, Dalian, Liaoning 116000, P.R. China.
Mol Med Rep. 2023 Jun;27(6). doi: 10.3892/mmr.2023.13010. Epub 2023 May 19.
Acute myocardial infarction (AMI) is a serious disease which threatens public health. Exosomes (exos) contain certain genetic information and are important communication vehicles between cells. In the present study, different exosomal microRNAs (miRs), which exhibit a notable association between expression levels in plasma and AMI were assessed to support the development of new diagnostic and clinical assessment markers of patients with AMI. In total, 93 individuals, including 31 healthy controls and 62 patients with AMI, were recruited for the present study. Data on age, blood pressure, glucose levels, lipid levels and coronary angiography images were collected from the enrolled individuals, and plasma samples were collected. Plasma exos were extracted and verified using ultracentrifugation, transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting (WB). Exo‑miR‑4516 and exo‑miR‑203 in plasma exos were identified by exosomal miRNA sequencing analysis, reverse transcription‑quantitative PCR was performed to detect the levels of exo‑miR‑4516 and exo‑miR‑203 in plasma exos, and ELISA was performed to detect the levels of secretory frizzled‑related protein 1 (SFRP1) in samples. The correlation analysis between exo‑miR‑4516, exo‑miR‑203 and SFRP1 in plasma exos and AMI was presented as receiver operating characteristic curves (ROCs) of the SYNTAX score, cardiac troponin I (cTnI), low‑density lipoprotein (LDL) and each indicator separately. Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed to predict relevant enrichment pathways. Exos were successfully isolated from plasma by ultracentrifugation, which was confirmed by TEM, NTA and WB. Exo‑miR‑4516, exo‑miR‑203 and SFRP1 levels in plasma were significantly higher in the AMI group compared with the healthy control group. ROCs demonstrated that exo‑miR‑4516, exo‑miR‑203 and SFRP1 levels had a high diagnostic efficiency in predicting AMI. Exo‑miR‑4516 was positively correlated with SYNTAX score, and plasma SFRP1 was positively correlated with plasma cTnI and LDL. In conclusion, the data demonstrated that exo‑miR‑4516, exo‑miR‑203 and SFRP1 levels could be used in combination to diagnose and assess the severity of AMI. The present study was retrospectively registered (TRN, NCT02123004).
急性心肌梗死（AMI）是一种严重威胁公众健康的疾病。外泌体（exos）包含特定的遗传信息，是细胞间重要的通讯载体。本研究评估了不同外泌体微小 RNA（miRs）在血浆中的表达水平与 AMI 之间存在显著相关性，为 AMI 患者的新型诊断和临床评估标志物的开发提供了支持。共纳入 93 例患者，包括 31 例健康对照和 62 例 AMI 患者，收集了参与者的年龄、血压、血糖水平、血脂水平和冠状动脉造影图像数据，并采集了血浆样本。使用超速离心、透射电子显微镜（TEM）、纳米颗粒跟踪分析（NTA）和 Western blot（WB）提取和验证血浆外泌体。通过外泌体 miRNA 测序分析鉴定血浆外泌体中的 exo-miR-4516 和 exo-miR-203，采用逆转录定量 PCR 检测血浆外泌体中 exo-miR-4516 和 exo-miR-203 的水平，采用 ELISA 检测样品中分泌卷曲相关蛋白 1（SFRP1）的水平。呈现了血浆外泌体中 exo-miR-4516、exo-miR-203 和 SFRP1 与 AMI 之间的相关性分析，作为 SYNTAX 评分、心肌肌钙蛋白 I（cTnI）、低密度脂蛋白（LDL）和各指标单独的受试者工作特征曲线（ROC）。京都基因与基因组百科全书富集分析用于预测相关富集途径。通过超速离心成功从血浆中分离出外泌体，通过 TEM、NTA 和 WB 进行了确认。与健康对照组相比，AMI 组患者的血浆外泌体中 exo-miR-4516、exo-miR-203 和 SFRP1 水平显著升高。ROC 表明，exo-miR-4516、exo-miR-203 和 SFRP1 水平在预测 AMI 方面具有较高的诊断效率。exo-miR-4516 与 SYNTAX 评分呈正相关，而血浆 SFRP1 与血浆 cTnI 和 LDL 呈正相关。总之，数据表明，exo-miR-4516、exo-miR-203 和 SFRP1 水平可联合用于诊断和评估 AMI 的严重程度。本研究为回顾性研究（TRN，NCT02123004）。
