Department of Orthopedics, The Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
Mol Med Rep. 2023 Jul;28(1). doi: 10.3892/mmr.2023.13017. Epub 2023 May 19.
In the field of orthopedics, defects in large bones have proven challenging to resolve. The aim of the present study was to address this problem through the combination of tantalum metal (pTa) with exosomes derived from bone marrow mesenchymal stem cells (BMSCs), which have the potential to enhance regeneration of full thickness femoral bone defects in rats. Cell culture results demonstrated that exosomes improved the proliferation and differentiation of BMSCs. Following establishment of a supracondylar femoral bone defect, exosomes and pTa were implanted into the defect area. Results demonstrated that pTa acts as a core scaffold for cell adhesion and exhibits good biocompatibility. Moreover, micro‑CT scan results as well as histological examination demonstrated that pTa had a significant effect on osteogenesis, with the addition of exosomes further promoting bone tissue regeneration and repair. In conclusion, this novel composite scaffold can effectively promote bone regeneration in large bone defect areas, providing a new approach for the treatment of large bone defects.
在骨科领域，大骨缺损的问题一直难以解决。本研究旨在通过将钽金属（pTa）与骨髓间充质干细胞（BMSCs）来源的外泌体相结合，来解决这个问题，从而促进大鼠全层股骨骨缺损的再生。细胞培养结果表明，外泌体可促进 BMSCs 的增殖和分化。建立髁上股骨骨缺损模型后，将外泌体和 pTa 植入缺损区域。结果表明，pTa 可作为细胞黏附的核心支架，具有良好的生物相容性。此外，Micro-CT 扫描结果和组织学检查表明，pTa 对成骨有显著作用，添加外泌体进一步促进了骨组织的再生和修复。综上所述，这种新型复合支架可有效促进大骨缺损区域的骨再生，为大骨缺损的治疗提供了一种新方法。
