Parasite Biology in the Amazon Region Graduate Program, Pará State University, Belém 66087-670, Brazil.
Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Health and Environment Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, Brazil.
Viruses. 2023 May 11;15(5):1150. doi: 10.3390/v15051150.
As part of a continuous effort to investigate the viral communities associated with wild mammals at the human-animal interface in an Amazonian metropolitan region, this study describes the detection of a novel rodent-borne arterivirus. A sample containing pooled organs of was submitted to RNA sequencing, and four sequences taxonomically assigned as related to the family were recovered, corresponding to an almost complete genome of nearly 13 kb summed. In the phylogenetic analysis with the standard domains used for taxa demarcation in the family, the tentatively named Oecomys arterivirus 1 (OAV-1) was placed within the clade of rodent- and porcine-associated viruses, corresponding to the subfamily. The divergence analysis, based on the same amino acid alignment, corroborated the hypothesis that the virus may represent a new genus within the subfamily. These findings contribute to the expansion of the current knowledge about the diversity, host and geographical range of the viral family. Arterivirids are non-human pathogens and are usually species-specific, but the susceptibility of cell lines derived from different organisms should be conducted to confirm these statements for this proposed new genus in an initial attempt to assess its spillover potential.
作为在亚马逊城市地区的人与动物接触界面上调查与野生动物相关的病毒群落的持续努力的一部分，本研究描述了一种新型啮齿动物传播的动脉病毒的检测。一份包含混合器官的样本被提交进行 RNA 测序，共回收了四个被分类为与 科相关的序列，对应于一个近 13 kb 长的几乎完整基因组。在使用家族分类标准域进行的系统发育分析中，暂命名为 Oecomys arterivirus 1 (OAV-1) 的病毒被放置在与啮齿动物和猪相关病毒的分支内，对应于 亚科。基于相同的氨基酸比对的分化分析证实了病毒可能代表亚科内的一个新属的假设。这些发现有助于扩展关于病毒科的多样性、宿主和地理范围的现有知识。动脉病毒是非人类病原体，通常是物种特异性的，但应进行来自不同生物体的细胞系的易感性研究，以确认这些说法适用于这个新提议的属，这是初步评估其溢出潜力的尝试。
