Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Planta. 2023 May 31;258(1):9. doi: 10.1007/s00425-023-04166-4.
NOR-like1 regulates tomato fruit size by targeting SlARF9, SlGRAS2, SlFW3.2, and SlFW11.3 genes involved in cell division and cell expansion. Fruit size is an important agricultural character that determines the yield of crops. Here, we found that NAC transcription factor NOR-like1 regulated fruit size by regulating cell layer number and cell area in tomato. Over-expressing NOR-like1 gene in tomato reduced fruit weight and size, whereas the knock-out of NOR-like1 increased fruit weight and size. At the molecular level, NOR-like1 binds to the promoter of SlGRAS2, SlFW3.2, and SlFW11.3 to repress their transcription, while it also binds to the promoter of ARF9 to activate its transcription. Overall, these results expand the biological function of NOR-like1 and deepen our understanding of the transcriptional network that regulates tomato fruit size.
NOR-like1 通过靶向 SlARF9、SlGRAS2、SlFW3.2 和 SlFW11.3 基因调控番茄果实大小，这些基因参与细胞分裂和细胞扩张。果实大小是决定作物产量的一个重要农业性状。在这里，我们发现 NAC 转录因子 NOR-like1 通过调节番茄果实中的细胞层数和细胞面积来调控果实大小。在番茄中过表达 NOR-like1 基因会降低果实重量和大小，而 NOR-like1 的敲除则会增加果实重量和大小。在分子水平上，NOR-like1 与 SlGRAS2、SlFW3.2 和 SlFW11.3 的启动子结合，抑制其转录，同时也与 ARF9 的启动子结合，激活其转录。总的来说，这些结果扩展了 NOR-like1 的生物学功能，加深了我们对调控番茄果实大小的转录网络的理解。
