Department of Biology, School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phaholyothin Road, Klong Luang, Pathum Thani, 12120, Thailand.
Int J Syst Evol Microbiol. 2023 Jun;73(6). doi: 10.1099/ijsem.0.005765.
An endophytic actinobacterium, designated strain PLAI 1-29, was isolated from the root tissue of collected from Pathum Thani province, Thailand. Strain PLAI 1-29 was characterized using a polyphasic taxonomic approach. It typically exhibited morphological and chemotaxonomic properties of the genus . Strain PLAI 1-29 produced a spiral spore chain on aerial mycelium and grew at 15-40 °C, pH 6-10 on International Project 2 agar. The maximum NaCl concentration for growth was 9 % (w/v). Cells of strain PLAI 1-29 presented -diaminopimelic acid, arabinose, galactose and ribose. The detected phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The major menaquinones were MK-9(H) and MK-9(H). The major cellular fatty acids were -C, -C and -C. The genome-based taxonomic details revealed the assignment of strain PLAI 1-29 to the genus and exhibited low threshold values for the delineation of a novel species by average nucleotide identity-blast (84.0%), average amino acid identity (80.0%) and digital DNA-DNA hybridization (27.6%) with its closest type strain, S187. Furthermore, several differential physiological and biochemical characteristics were detected between strain PLAI 1-29 and the closest type strain. Based on the combined phenotypic and genomic features, strain PLAI 1-29 (=TBRC 7645=NBRC 113170) is considered to represent a new species, for which we propose the name sp. nov.
一株内生放线菌，被命名为 PLAI 1-29 菌株，从泰国巴吞他尼府采集的根组织中分离得到。PLAI 1-29 菌株通过多相分类学方法进行了表征。它通常表现出属 的形态学和化学分类学特性。PLAI 1-29 菌株在气生菌丝上产生螺旋孢子链，并在 International Project 2 琼脂上生长，温度为 15-40°C，pH 值为 6-10。最大生长盐浓度为 9%（w/v）。PLAI 1-29 菌株的细胞含有二氨基庚二酸、阿拉伯糖、半乳糖和核糖。检测到的磷脂有双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。主要的menaquinones 是 MK-9(H)和 MK-9(H)。主要的细胞脂肪酸是 -C、-C 和 -C。基于基因组的分类学细节表明，PLAI 1-29 菌株被分配到属 ，并且与最接近的模式菌株 S187 相比，平均核苷酸同一性-blast（84.0%）、平均氨基酸同一性（80.0%）和数字 DNA-DNA 杂交（27.6%）的阈值较低，表明其可以划分为一个新的种。此外，在 PLAI 1-29 菌株和最接近的模式菌株之间还检测到了几种不同的生理生化特征。基于表型和基因组特征的综合分析，PLAI 1-29 菌株（=TBRC 7645=NBRC 113170）被认为代表了一个新的 种，我们提议将其命名为 sp. nov.
