School of Chemical and Physical Sciences, Keele University, Keele, UK.
Cranfield Forensic Institute, Cranfield University, Shrivenham, UK.
Sci Rep. 2023 Jun 8;13(1):9331. doi: 10.1038/s41598-023-33547-8.
Ductal carcinoma in-situ (DCIS) accounts for 20-25% of all new breast cancer diagnoses. DCIS has an uncertain risk of progression to invasive breast cancer and a lack of predictive biomarkers may result in relatively high levels (~ 75%) of overtreatment. To identify unique prognostic biomarkers of invasive progression, crystallographic and chemical features of DCIS microcalcifications have been explored. Samples from patients with at least 5-years of follow up and no known recurrence (174 calcifications in 67 patients) or ipsilateral invasive breast cancer recurrence (179 microcalcifications in 57 patients) were studied. Significant differences were noted between the two groups including whitlockite relative mass, hydroxyapatite and whitlockite crystal maturity and, elementally, sodium to calcium ion ratio. A preliminary predictive model for DCIS to invasive cancer progression was developed from these parameters with an AUC of 0.797. These results provide insights into the differing DCIS tissue microenvironments, and how these impact microcalcification formation.
导管原位癌（DCIS）占所有新诊断乳腺癌的 20-25%。DCIS 向浸润性乳腺癌进展的风险不确定，缺乏预测性生物标志物可能导致过度治疗的比例相对较高（约 75%）。为了确定浸润性进展的独特预后生物标志物，已经探索了 DCIS 微钙化的晶体学和化学特征。对至少有 5 年随访且无已知复发（67 例患者中有 174 个钙化灶）或同侧浸润性乳腺癌复发（57 例患者中有 179 个微钙化灶）的患者进行了研究。两组之间存在显著差异，包括羟磷灰石相对质量、羟基磷灰石和羟磷灰石晶体成熟度以及元素钠钙离子比。从这些参数中开发了一个用于预测 DCIS 向浸润性癌进展的初步预测模型，AUC 为 0.797。这些结果提供了对不同 DCIS 组织微环境的深入了解，以及这些微环境如何影响微钙化的形成。
