Chair of Microbiology, Technical University of Munich, Gregor-Mendel-Straße 4, 85354 Freising, Germany.
Int J Syst Evol Microbiol. 2023 Jun;73(6). doi: 10.1099/ijsem.0.005927.
Four strains of members of the genus were isolated from samples associated with the western honey bee , which could not be assigned to a species with a validly published name. Strains TMW 2.2543, TMW 2.2556, TMW 2.2558 and TMW 2.2559 exhibit DNA-DNA hybridisation (isDDH) and orthologous average nucleotide identity (orthoANI) values below species delineation thresholds compared with all described species of the genus and with each other. TMW 2.2556 and TMW 2.2558 form their own clade within the genus. The major respiratory quinone of all strains was Q-10. The composition of cellular fatty acids was diverse between strains. All strains stained Gram-negative, were rod-shaped, strictly aerobic, pellicle-forming, catalase-positive, oxidase-negative, mesophilic and grew over a wide pH range; they were halosensitive but glucose-tolerant. Unlike the other studied strains, TMW 2.2558 was non-motile. Phylogenetic, chemotaxonomic and physiological analyses revealed a clear distinction between all the strains and species with validly published names. All the data support the proposition of four novel species within the genus , namely sp. nov., sp. nov., sp. nov. and sp. nov., with the respective type strains sp. nov. TMW 2.2543 (= DSM 114872, = LMG 32791), sp. nov. TMW 2.2556 (= DSM 114874, = LMG 32792), sp. nov. TMW 2.2558 (= DSM 114875, = LMG 32793) and sp. nov. TMW 2.2559 (= DSM 114877, = LMG 32794). Moreover, three genomes available in the NCBI database that have not yet been described as species with validly published names could be assigned to the proposed species. sp. ESL0378 and sp. ESL0385 to sp. nov. and sp. AS1 to sp. nov.
从与西方蜜蜂相关的样本中分离出了四个属的菌株，这些菌株无法被分配到具有有效发表名称的物种。菌株 TMW 2.2543、TMW 2.2556、TMW 2.2558 和 TMW 2.2559 的 DNA-DNA 杂交（isDDH）和直系同源平均核苷酸同一性（orthoANI）值与属内所有描述的物种以及彼此相比均低于物种划分阈值。菌株 TMW 2.2556 和 TMW 2.2558 在属内形成自己的分支。所有菌株的主要呼吸醌均为 Q-10。细胞脂肪酸的组成在菌株之间差异很大。所有菌株均革兰氏阴性、杆状、严格需氧、形成菌膜、过氧化氢酶阳性、氧化酶阴性、嗜中温且在较宽的 pH 范围内生长；它们对盐敏感但能耐受葡萄糖。与其他研究的菌株不同，菌株 TMW 2.2558 不运动。系统发育、化学分类和生理分析表明，所有菌株与具有有效发表名称的物种之间存在明显区别。所有数据均支持在属内提出四个新种，即 sp. nov.、 sp. nov.、 sp. nov. 和 sp. nov.，其相应的模式菌株分别为 sp. nov. TMW 2.2543（=DSM 114872，=LMG 32791）、 sp. nov. TMW 2.2556（=DSM 114874，=LMG 32792）、 sp. nov. TMW 2.2558（=DSM 114875，=LMG 32793）和 sp. nov. TMW 2.2559（=DSM 114877，=LMG 32794）。此外，可将 NCBI 数据库中尚未被描述为具有有效发表名称的物种的三个基因组分配给所提议的物种。基因组 ESL0378 和 ESL0385 分别被分配给 sp. nov.和 sp. nov.，而基因组 AS1 被分配给 sp. nov.。
