Guzman Juan, Won Miyoung, Poehlein Anja, Sombolestani Atena Sadat, Mayorga-Ch Daniela, Laureys David, Clippeleer Jessika De, Kämpfer Peter, Daniel Rolf, Vilcinskas Andreas, Vandamme Peter, Kwon Soon-Wo
Department of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Giessen, Germany.
Institute for Insect Biotechnology, Justus-Liebig-University of Giessen, Giessen, Germany.
Int J Syst Evol Microbiol. 2023 Feb;73(2). doi: 10.1099/ijsem.0.005699.
Acetic acid bacteria (family ) are found in the gut of most insects. Two clades are currently recognized: and a-. The latter group is only found in hymenopteran insects and the described species have been isolated from bees and ants. In this study, two new strains DDB2-T1 (=KACC 21507=LMG 31759) and DM15PD (=CCM 9165=DSM 112731=KACC 22353=LMG 32454) were isolated from wasps collected in the Republic of Korea and Germany, respectively. Molecular and phenotypic analysis revealed that the strains are closely related, with 16S rRNA gene sequences showing 100 % identity and genomic average nucleotide identity (ANI) values ≥99 %. The closest related species based on type strain 16S rRNA gene sequences are , , and (94.8-94.7% identity), whereas the closest related species based on type strain genome analysis are and (ANI values of 68.8 and 68.2 %, respectively). The reconstruction of a phylogenomic tree based on 107 core proteins revealed that the branch leading to DDB2-T1 and DM15PD is localized between and . Further genomic distance metrics such as ANI, percentage of conserved proteins and alignment fraction values were consistent with these strains belonging to a new genus. The key phenotypic characteristics were one MALDI-TOF-MS peak (m/z=4601.9±2.0) and the ability to produce acid from d-arabinose. Based on this polyphasic approach, including phylogenetics, phylogenomics, genome distance calculations, ecology and phenotypic characteristics, we propose to name the novel strains gen. nov., sp. nov., with the type strain DDB2-T1 (=KACC 21507=LMG 31759).
醋酸菌（科）存在于大多数昆虫的肠道中。目前已识别出两个进化枝： 和a-。后一组仅在膜翅目昆虫中发现，且已描述的物种是从蜜蜂和蚂蚁中分离出来的。在本研究中，分别从韩国和德国采集的黄蜂中分离出两株新菌株DDB2-T1（=KACC 21507=LMG 31759）和DM15PD（=CCM 9165=DSM 112731=KACC 22353=LMG 32454）。分子和表型分析表明，这些菌株密切相关，16S rRNA基因序列显示100%的同一性，基因组平均核苷酸同一性（ANI）值≥99%。基于模式菌株16S rRNA基因序列，最密切相关的物种是 、 、 和 （同一性为94.8 - 94.7%），而基于模式菌株基因组分析，最密切相关的物种是 和 （ANI值分别为68.8和68.2%）。基于107个核心蛋白重建的系统发育基因组树表明，通向DDB2-T1和DM15PD的分支位于 和 之间。进一步的基因组距离指标，如ANI、保守蛋白百分比和比对分数值，与这些菌株属于一个新属一致。关键的表型特征是一个基质辅助激光解吸电离飞行时间质谱峰（m/z = 4601.9±2.0）以及从D-阿拉伯糖产酸的能力。基于这种多相方法，包括系统发育学、系统发育基因组学、基因组距离计算、生态学和表型特征，我们提议将新菌株命名为 属， 种，模式菌株为DDB2-T1（=KACC 21507=LMG 31759）。
