Sugar Beet Seed Institute (SBSI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Sci Rep. 2023 Jun 20;13(1):10019. doi: 10.1038/s41598-023-37217-7.
The methods utilized to analyze genotype by environment interaction (GEI) and assess the stability and adaptability of genotypes are constantly changing and developing. In this regard, often instead of depending on a single analysis, it is better to use a combination of several methods to measure the nature of the GEI from various dimensions. In this study, the GEI was investigated using different methods. For this purpose, 18 sugar beet genotypes were evaluated in randomized complete block design in five research stations over 2 years. The additive effects analysis of the additive main effects and multiplicative interaction (AMMI) model showed that the effects of genotype, environment and GEI were significant for root yield (RY), white sugar yield (WSY), sugar content (SC), and extraction coefficient of sugar (ECS). The multiplicative effect's analysis of AMMI into interaction principal components (IPCs) showed that the number of significant components varies from one to four in the studied traits. According to the biplot of the mean yield against the weighted average of absolute scores (WAAS) of the IPCs, G2 and G16 for RY, G16 and G2 for WSY, G6, G4, and G1 for SC and G8, G10 and G15 for ECS were identified as stable genotypes with optimum performance. The likelihood ratio test showed that the effects of genotype and GEI was significant for all studied traits. In terms of RY and WSY, G3 and G4 had high mean values of the best linear unbiased predictions (BLUP), so they were identified as suitable genotypes. However, in terms of SC and ECS, G15 obtained high mean values of the BLUP. The GGE biplot method classified environments into four (RY and ECS) and three (WSY and SC) mega-environments (MEs). Based on the multi-trait stability index (MTSI), G15, G10, G6, and G1 were the most ideal genotypes.
利用分析基因型与环境互作(GEI)的方法,并评估基因型的稳定性和适应性正在不断变化和发展。在这方面,通常最好不是依赖于单一的分析,而是使用几种方法的组合,从不同的维度来衡量 GEI 的性质。在本研究中,使用不同的方法来研究 GEI。为此,18 个甜菜基因型在 2 年的 5 个研究站中以随机完全区组设计进行了评估。加性主效和互作分析(AMMI)模型的加性效应分析表明,基因型、环境和 GEI 对块根产量(RY)、白蔗糖产量(WSY)、含糖量(SC)和糖提取系数(ECS)的影响显著。AMMI 中互作主成分(IPC)的乘性效应分析表明,在所研究的性状中,显著成分的数量从一个到四个不等。根据均值产量相对于 IPC 的加权平均绝对得分(WAAS)的双标图,对于 RY,G2 和 G16;对于 WSY,G16 和 G2;对于 SC,G6、G4 和 G1;对于 ECS,G8、G10 和 G15 被鉴定为具有最佳性能的稳定基因型。似然比检验表明,基因型和 GEI 的效应在所有研究性状中均显著。在 RY 和 WSY 方面,G3 和 G4 具有最佳线性无偏预测(BLUP)的高均值,因此被鉴定为合适的基因型。然而,在 SC 和 ECS 方面,G15 获得了高的 BLUP 均值。GGE 双标图法将环境分为四个(RY 和 ECS)和三个(WSY 和 SC)大环境(MEs)。根据多性状稳定性指数(MTSI),G15、G10、G6 和 G1 是最理想的基因型。